The process of repeat-induced point mutations (RIP) was used to disrupt the gene encoding the 80-kDa heat-inducible protein of Neurospora crassa. Germinated conidia of the wild-type recipient strain were electrotransformed with a plasmid containing a 7-kb fragment harbouring the complete hsp80 gene sequence. Some of the transformants with a duplication of hsp80 gene sequence showed extensive methylation of these sequences even in vegetatively growing cells. The presence of an extra gene copy in transformants of this type resulted in a marked reduction in the expression of this gene. Progeny of a cross of one such transformant, showing methylation of hsp80, was analyzed by Southern blot and Northern blot hybridization to examine the relationship between methylation and the accumulation of hsp80 mRNA under hyperthermia. In addition, HSP80 polypeptide levels were monitored in stressed and unstressed cells by immunoblot analysis using polyclonal anti-HSP80 IgG preparations. A correlation between the extent of RIP and expression of this gene was observed in the progeny isolates.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1139/o96-005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Regulatory Effect of Polysaccharides on Intestinal Microbiota and Metabolites during Fermentation.
J Agric Food Chem
March 2024
College of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, China.
polysaccharides (HSPs) have attracted extensive attention recently for their biological activity and physicochemical property. This research investigated the extraction, structural characterization, and prebiotic activity of three different HSPs (HSP40-0, HSP60-0, and HSP80-0) to reveal the scientific support for the high-value utilization of HSPs were heteropolysaccharide with diverse structures and surface morphologies. Comprehensive analysis was conducted through 16S rRNA gene sequencing and metabolite profiling techniques, and results showed that HSPs had different potentials to regulate the gut microbiota due to their different structures; for instance, both HSP40-0 and HSP80-0 could notably increase the relative abundance of Bacteroidota, whereas HSP60-0 could increase the relative abundance of .
View Article and Find Full Text PDFStHsfB5 Promotes Heat Resistance by Directly Regulating the Expression of Genes in Potato.
Int J Mol Sci
November 2023
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
With global warming, high temperatures have become a major environmental stress that inhibits plant growth and development. Plants evolve several mechanisms to cope with heat stress accordingly. One of the important mechanisms is the Hsf (heat shock factor)-Hsp (heat shock protein) signaling pathway.
View Article and Find Full Text PDFCalmodulin, Calcium/Calmodulin-Dependent Kinases-1 and 2 Regulate Expression of the Heat Shock Proteins for Heat Shock Tolerance and Pheromone Signaling Genes for Sexual Development in .
Indian J Microbiol
September 2023
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781 039 India.
Unlabelled: Calmodulin (CaM) is a primary Ca sensor that binds and activates numerous target proteins and regulates several cellular processes in eukaryotes. CaM is essential in ; therefore, we generated a CaM mutant using repeat-induced point (RIP) mutation and investigated the mutant phenotypes. We also studied knockout mutants of four Ca/CaM kinases (, , , and ) for their role during stress conditions and sexual development.
View Article and Find Full Text PDFThe cell functions of phospholipase C-1, Ca/H exchanger-1, and secretory phospholipase A in tolerance to stress conditions and cellulose degradation in Neurospora crassa.
Arch Microbiol
September 2023
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781 039, India.
We investigated the cell functions of the Ca signaling genes phospholipase C-1 (plc-1), Ca/H exchanger (cpe-1), and secretory phospholipase A (splA) for stress responses and cellulose utilization in Neurospora crassa. The Δplc-1, Δcpe-1, and ΔsplA mutants displayed increased sensitivity to the alkaline pH and reduced survival during induced thermotolerance. The ΔsplA mutant also exhibited hypersensitivity to the DTT-induced endoplasmic reticulum (ER) stress, increased microcrystalline cellulose utilization, increased protein secretion, and glucose accumulation in the culture supernatants.
View Article and Find Full Text PDFRice DST transcription factor negatively regulates heat tolerance through ROS-mediated stomatal movement and heat-responsive gene expression.
Front Plant Sci
January 2023
Key Laboratory of Specialty Agri-product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou, China.
Plants are frequently subjected to a broad spectrum of abiotic stresses including drought, salinity and extreme temperatures and have evolved both common and stress-specific responses to promote fitness and survival. Understanding the components and mechanisms that underlie both common and stress-specific responses can enable development of crop plants tolerant to different stresses. Here, we report a rice () mutant with increased heat tolerance.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!