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Integrated multi-omics reveals the molecular mechanisms underlying efficient phosphorus use under phosphate deficiency in elephant grass ().
Front Plant Sci
December 2022
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Phosphorus (P) is an essential macronutrient element for plant growth, and deficiency of inorganic phosphate (Pi) limits plant growth and yield. Elephant grass () is an important fodder crop cultivated widely in tropical and subtropical areas throughout the world. However, the mechanisms underlying efficient P use in elephant grass under Pi deficiency remain poorly understood.
View Article and Find Full Text PDFGenome-wide survey of the phosphofructokinase family in cassava and functional characterization in response to oxygen-deficient stress.
BMC Plant Biol
August 2021
College of Tropical Crops, Hainan University, Haikou, 570228, P. R. China.
Background: Glycolytic pathway is common in all plant organs, especially in oxygen-deficient tissues. Phosphofructokinase (PFK) is a rate-limiting enzyme in the glycolytic pathway and catalyses the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Cassava (M.
View Article and Find Full Text PDFGenome-scale revealing the central metabolic network of the fast growing methanotroph Methylomonas sp. ZR1.
World J Microbiol Biotechnol
January 2021
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin, 300308, China.
Methylomonas sp. ZR1 was an isolated new methanotrophs that could utilize methane and methanol growing fast and synthesizing value added compounds such as lycopene. In this study, the genomic study integrated with the comparative transcriptome analysis were taken to understanding the metabolic characteristic of ZR1 grown on methane and methanol at normal and high temperature regime.
View Article and Find Full Text PDFHeterologous expression and kinetic characterization of the α, β and αβ blend of the PPi-dependent phosphofructokinase from Citrus sinensis.
Plant Sci
March 2019
Instituto de Agrobiotecnología del Litoral (IAL, CONICET-UNL) & FBCB, Centro Científico Tecnológico CONICET Santa Fe, Colectora Ruta Nac. Nº 168 km. 0, Paraje El Pozo, S3000ZAA, Santa Fe, Argentina. Electronic address:
This work reports the molecular cloning and heterologous expression of the genes coding for α and β subunits of pyrophosphate-dependent phosphofructokinase (PPi-PFK) from orange. When expressed individually, both recombinant subunits were produced as highly purified monomeric proteins able to phosphorylate fructose-6-phosphate at the expenses of PPi (specific activity of 0.075 and 0.
View Article and Find Full Text PDFN-Terminal Presequence-Independent Import of Phosphofructokinase into Hydrogenosomes of Trichomonas vaginalis.
Eukaryot Cell
December 2015
Department of Parasitology, Charles University in Prague, Faculty of Science, Prague, Czech Republic
Mitochondrial evolution entailed the origin of protein import machinery that allows nuclear-encoded proteins to be targeted to the organelle, as well as the origin of cleavable N-terminal targeting sequences (NTS) that allow efficient sorting and import of matrix proteins. In hydrogenosomes and mitosomes, reduced forms of mitochondria with reduced proteomes, NTS-independent targeting of matrix proteins is known. Here, we studied the cellular localization of two glycolytic enzymes in the anaerobic pathogen Trichomonas vaginalis: PPi-dependent phosphofructokinase (TvPPi-PFK), which is the main glycolytic PFK activity of the protist, and ATP-dependent PFK (TvATP-PFK), the function of which is less clear.
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