AI Article Synopsis
- The paper discusses the analysis of specific compounds (C16-AGEPC and C18-AGEPC) found in human blood using a technique called micellar electrokinetic capillary chromatography (MECC).
- The optimal conditions for this analysis were established, including the composition of the running buffer and achieving separation within 10 minutes.
- The method showed reliable detection limits and precision, making it suitable for clinical sample analysis.
Article Abstract
In this paper, 1-O-hexadecyl and 1-O-octadecyl-2-acetyl-sn-glycero-3- phosphorylcholine (C16-AGEPC and C18-AGEPC) in the platelet activating factor of human blood were analyzed by micellar electrokinetic capillary chromatography (MECC) with indirect ultraviolet absorption detection at 254 nm. The optimum running buffer for the separation contained 50 mmol/l sodium dodecy sulphate, 20 mmol/l potassium hydrogen phthalate, 10 mmol/l borax and 3 mol/l urea (pH 6.8). The separation was completed within 10 min. The detection limits of C16-AGEPC and C18-AGEPC were the same, i.e. 60 ng/ml (k = 3). The analytical precision (n = 6) was 2.8-3.0% and 1.4-1.7% for the determination (peak height mode) and for the measurement of the migration times, respectively. The application of this method to the clinical samples was demonstrated.
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