Pepsin secretion was studied in vivo in the primary culture of isolated secretory gastric mucosa cells of the frog (Rana ridibunda). The injection of animals with carbacholin (200 micrograms/100 g weight) or histamine (100 micrograms/100 g weight) or addition of the hormones (100 microM) into the incubation medium of isolated gastric cells significantly increased pepsin activity. In vitro, preliminary addition of the specific antagonists of these hormones to the incubation medium (1 microM atropine or 100 microM cimetidine) completely suppressed the effects of hormones. The effect of histamine is season-dependent. Combined injection or simultaneous addition to incubation medium of isolated cells of carbacholin and histamine caused mutual potentiation of their effects. In this case, the quantitative response of the cells exceeded the theoretical sum of the stimulatory effects of each of these two hormones alone. According to electrophoresis, the hormones cause quantitative changes in isozyme spectrum of proteolytic enzymes but do not significantly affect their qualitative characteristics.
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