AI Article Synopsis
- An enrichment cloning method was used to isolate microsatellite loci from eastern white pine, achieving significant enrichment for (AC)n repeats compared to (ACAG)n repeats.
- From 77 primer pairs, 19 microsatellite loci were successfully identified, with genotyping revealing an average of 5.4 alleles and 0.515 observed heterozygosity across 16 unrelated pines.
- The study confirmed that (AC)n loci are highly variable and highlighted the effective development of SSR markers for use in genome mapping and population genetics research.
Article Abstract
An enrichment cloning method was evaluated for the isolation of microsatellite loci from eastern white pine and the resulting markers were examined for polymorphisms. A 200-fold enrichment was achieved for highly abundant (AC)n repeats, but for much less abundant (ACAG)n repeats an enrichment of only 20-fold was obtained. Using a single set of PCR conditions, 19 microsatellite loci were identified from 77 primer pairs evaluated. Genotyping of 16 (AC)n loci in 16 unrelated white pines from the north-central United States revealed an average of 5.4 alleles per locus and an average observed heterozygosity of 0.515. Five loci were scored among megagametophytes from a single pine to obtain a haploid genotype of the segregating female meiotic products. All loci segregated according to Mendelian expectations and linkage was established for two of the loci. It was concluded that (AC)n loci are highly variable in this species and that SSR (simple sequence repeat) markers can be efficiently developed for genome mapping and population genetics studies.
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| http://dx.doi.org/10.1139/g96-138 | DOI Listing |
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