AI Article Synopsis
- Computer methods revealed strong similarities in amino-acid sequences among 21 amidases, all of which help break down organic nitrogen compounds and produce ammonia.
- A conserved motif was identified that could play a key role in how these enzymes bind to amides and function catalytically.
- The study also highlighted how genetic transfer mechanisms, like transposons and plasmids, may have helped spread these genes, providing insights into the evolution of these and related enzymes.
Article Abstract
Computer methods for database search, multiple alignment and cluster analysis indicated significant homology between amino-acid sequences of 21 amidases or amidohydrolases (EC 3.5). All of them were found to be involved in the reduction of organic nitrogen compounds and ammonia production. A conserved motif was found which may be important in amide binding and in catalytic mechanisms. Homology studies between these amidases and some ureases, nitrilases and acyl-transferases or enzymes with unknown functions provided new insight into the evolution of these proteins. Dissemination of these genes seemed to be facilitated by transfer of genetic elements such as transposons and plasmids.
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| http://dx.doi.org/10.1016/s0167-4838(96)00145-8 | DOI Listing |
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