To study the MR appearance of knee joint hyaline cartilage, 120 subjects were examined with MRI of the knee. Axial SE proton-density (PD) and T2, GE T1 and GE-MTC sequences were used, as well as coronal SE T1 and sagittal SE PD and T2 sequences. At the patellar and femorotibial cartilages the following variables were investigated: thickness, surface, signal intensity and visibility. Moreover, the three patellar sequences were compared qualitatively, by giving a 1-to-4 score to the images, depending on their yield in the four variables. As for articular cartilage thickness, a statistically significant difference was found between men and women in nearly all measurement sites, the mean values being always higher in men. A statistically significant inverse correlation between cartilage thickness and age was found, in men only, at the three load-bearing regions of the medial femoral condyle. Cartilage surface was irregular and/or its signal intensity altered in 28% of patients at the femoral condyles, while the cartilage of tibial plates exhibited normal surface and homogeneous signal intensity in most cases. The visibility rate of trilaminar cartilage was 82.5% for the medial femoral condyle, 87.5% for the lateral femoral condyle, 70% for the patella, 12.5% for the medial tibial plateau and 14.5% for the lateral tibial plateau. No statistically significant correlation was found between cartilage thickness, surface, signal intensity and visibility and age, sex, and body weight. The GE sequence was the best tool to study cartilage thickness and signal intensity, while the SE T2 sequence was the most accurate one to depict the articular surface and, together with the PD SE sequence, to visualize the trilaminar structure. Our study suggests that articular cartilage surface, thickness, and signal intensity can be studied accurately with SE PD and T2 sequences, combined with a GE T1 sequence.
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Mini Rev Med Chem
January 2025
Dipartimento di Farmacia, Università di Pisa, Via Bonanno 33, 56126, Pisa, Italia.
Luminescent Lanthanide (III) (Ln(III)) bioprobes (LLBs) have been extensively used in the last two decades as intracellular molecular probes in bio-imaging for the efficient revelation of analytes, to signal intracellular events (enzymes/protein activity, antigen-antibody interaction), target specific organelles, and determine parameters of particular biophysical interest, to gain important insights on pathologies or diseases. The choice of using a luminescent Ln(III) coordination compound with respect to a common organic fluorophore is intimately connected to how their photophysical sensitization (antenna effect) can be finely tuned and especially triggered to respond (even quantitatively) to a certain biophysical event, condition or analyte. While there are other reviews focused on how to design chromophoric ligands for an efficient sensitization of Ln(III) ions, both in the visible and NIR region, this review is application-driven: it is a small collection of particularly interesting examples where the LLB's emissive information is acquired by imaging the emission intensity and/or the fluorescence lifetime (fluorescence lifetime imaging microscopy, FLIM).
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January 2025
Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Universidade Federal de Viçosa. ZIP, 36.570-900, Viçosa - MG, Brazil.
Repetitive sequences are recognized for their roles in plant genome organization and function. Mobile elements are notable repeatome sequences due to their intrinsic mutagenic potential, which is related to the origin of adaptive novelties. Understanding the genomic organization and dynamics of the repeatome is fundamental to enlighten their role in plant genome evolution.
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January 2025
NanoBioMedical Centre, Adam Mickiewicz University, Wszechnicy Piastowskiej 3, 61-614, Poznań, Poland. Electronic address:
Plasmonic surface lattice resonance (SLR) is a phenomenon in which individual localized surface plasmon resonances (LSPRs) excited in periodically-arranged plasmonic nanoparticles couple through the interaction with the propagating diffracted incident light. The SLR optical absorption peak is by at least one order of magnitude more intense than the LSPR one, making SLR superior for applications in which LSPR is commonly used. Recently, we have developed a route for the fabrication of spherical virus-like particles (VLPs) with plasmonic Au cores and protein coronas, where the LSPR in the cores amplifies vibrational Raman signals originating from protein-antibody interactions [ACS Synth.
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January 2025
Uganda Cancer Institute, Department of Radiotherapy, Kampala, Uganda.
The evolution of radiation therapy in Uganda has been a journey marked by significant milestones and persistent challenges. Since the inception of radiotherapy services in 1988-1989, there has been a concerted effort to enhance cancer treatment services. The early years were characterized by foundational developments, such as the installation of the first teletherapy units, low-dose-rate brachytherapy units, and conventional simulators, and the recognition of radiation oncologists and medical physicist professionals laid the groundwork for radiotherapy treatment modalities.
View Article and Find Full Text PDFAnal Chem
January 2025
Department of Chemistry, Faculty of Science, Masaryk University, Brno 625 00, Czech Republic.
Obtaining high-quality matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) images and the reproducibility of the technique depend strongly on the sample preparation protocol. The most crucial part is the application of the MALDI matrix, which often relies on expensive spraying or sublimation coaters. In this work, we present a new dual-polarity matrix for MALDI mass spectrometry imaging (MSI): Basic Blue 7 (BB7), which belongs to the group of triarylmethane dyes.
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