A DNA fragment was identified and cloned from Staphylococcus epidermidis (Se) using femA from S. aureus (Sa) as a heterologous hybridization probe. DNA sequence analysis of a portion of this clone revealed two complete ORFs highly related to femA and femB of Sa. The genomic arrangement of the Se femA/B complex was nearly identical to that observed in Sa. Intra- and interspecies relatedness of these genes and conservation of genomic organization were consistent with gene duplication of one of these genes in an ancestral organism. Recombinant FEMA, produced in Escherichia coli (Ec), was purified to near homogeneity. Identity of the purified protein was verified by N-terminal amino acid (aa) sequence analysis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0378-1119(96)00450-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Diclofenac Resensitizes Methicillin-Resistant to -Lactams and Prevents Implant Infections.
Adv Sci (Weinh)
July 2021
Department of Bone and Joint Surgery, Department of Orthopedics, Renji Hospital, School of Medicine Shanghai Jiaotong University Shanghai 200127 China.
Article Synopsis
- * Low-dose diclofenac can enhance the effectiveness of traditional antibiotics, like β-lactams, and prevent MRSA from forming harmful biofilms on implants by suppressing specific genes linked to antibiotic resistance and biofilm formation.
- * Combining low-dose diclofenac with β-lactams shows promising results in reducing MRSA infections in animal models, suggesting potential for improved perioperative infection prevention strategies.
Partition of tRNAGly isoacceptors between protein and cell-wall peptidoglycan synthesis in Staphylococcus aureus.
Nucleic Acids Res
January 2021
INSERM, Sorbonne Université, Université de Paris, Centre de Recherche des Cordeliers (CRC), F-75006 Paris, France.
The sequence of tRNAs is submitted to evolutionary constraints imposed by their multiple interactions with aminoacyl-tRNA synthetases, translation elongation factor Tu in complex with GTP (EF-Tu•GTP), and the ribosome, each being essential for accurate and effective decoding of messenger RNAs. In Staphylococcus aureus, an additional constraint is imposed by the participation of tRNAGly isoacceptors in the addition of a pentaglycine side chain to cell-wall peptidoglycan precursors by transferases FmhB, FemA and FemB. Three tRNAGly isoacceptors poorly interacting with EF-Tu•GTP and the ribosome were previously identified.
View Article and Find Full Text PDFPrevalence of resistance and toxin genes in community-acquired and hospital-acquired methicillin-resistant clinical isolates.
Iran J Basic Med Sci
October 2020
Microbiology Department, Faculty of Pharmacy, Misr International University, Cairo, Egypt.
Objectives: Methicillin-resistant (MRSA) is one of the major health hazards and became of greater public health concern since the emergence of community-acquired MRSA. This work aimed to study the prevalence of , , , -PV, -PV (PVL), I, and II genes among community-acquired (CA) hospital-acquired (HA) MRSA to increase vigilance in the diagnosis and management of suspected infections.
Materials And Methods: isolates recovered from clinical samples were classified into community or hospital-acquired and tested for their antibiotic susceptibility against 19 antibiotics.
Expression of genes encoding resistance in Staphylococcus spp. isolated from bovine subclinical mastitis in Brazil.
J Infect Dev Ctries
July 2020
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechnics, University of São Paulo (USP), São Paulo, Brazil.
Introduction: Staphylococci are the most important agents associated with bovine mastitis. This study aimed at characterizing resistance factors to antimicrobials in Staphylococcus spp. isolated from the milk of cows with subclinical mastitis.
View Article and Find Full Text PDFFmhA and FmhC of incorporate serine residues into peptidoglycan cross-bridges.
J Biol Chem
September 2020
Department of Microbiology, University of Chicago, Chicago, Illinois, USA; Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Lemont, Illinois, USA. Electronic address:
Staphylococcal peptidoglycan is characterized by pentaglycine cross-bridges that are cross-linked between adjacent wall peptides by penicillin-binding proteins to confer robustness and flexibility. In , pentaglycine cross-bridges are synthesized by three proteins: FemX adds the first glycine, and the homodimers FemA and FemB sequentially add two Gly-Gly dipeptides. Occasionally, serine residues are also incorporated into the cross-bridges by enzymes that have heretofore not been identified.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!