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Antibodies and Inflammation: Fecal Biomarkers of Gut Health in Domestic Ruminants.
J Exp Zool A Ecol Integr Physiol
January 2025
Department of Earth and Environmental Science, School of Natural Sciences, Faculty of Science and Engineering, The University of Manchester, Manchester, UK.
Gastrointestinal infections present major challenges to ruminant livestock systems, and gut health is a key constraint on fitness, welfare, and productivity. Fecal biomarkers present opportunities to monitor animal health without using invasive methods, and with greater resolution compared to observational metrics. Here we developed enzyme-linked immunosorbent assays for three potential fecal biomarkers of gut health in domestic ruminants: two immunological (total immunoglobulin [Ig]A and total IgG) and one inflammatory (lactoferrin).
View Article and Find Full Text PDFEffect of Extraction Methods and Preheat Treatments on the Functional Properties of Pumpkin Seed Protein Concentrate.
ACS Food Sci Technol
January 2025
Department of Food Engineering, Middle East Technical University, Ankara 06800, Turkey.
This study explores the effect of different extraction methods and preheat treatments in obtaining protein concentrate from pumpkin seed flour. The effects on the yield and functional properties of pumpkin seed protein concentrate (PSPC) were compared alongside microwave and conventional preheating methods using alkali, salt, and enzyme-assisted alkali extraction techniques. Analytical assessments included proximate analysis, soluble protein content, water solubility index (WSI), emulsification activity (EA) and stability (ES), foaming capacity (FC) and stability (FS), and antioxidant activity (AA).
View Article and Find Full Text PDFAdvancements in surface-enhanced femtosecond stimulated Raman spectroscopy: exploring factors influencing detectability and shapes of spectra.
Nanophotonics
January 2025
Institute of Physical Chemistry, Polish Academy of Sciences, 01-224 Warsaw, Kasprzaka 44/52, Poland.
A combination of femtosecond stimulated Raman scattering and surface-enhanced Raman scattering, termed surface-enhanced stimulated Raman scattering (SE-FSRS), was proposed to leverage both temporal precision and sensitivity for advanced molecular dynamics analysis. During the initial successful implementations of this approach, unexpected spectral distortions were observed, and several potential explanations were proposed. Further progress in this novel technique and its broader implementation requires a profound understanding of the factors influencing the shape of the registered spectra and the underlying mechanisms.
View Article and Find Full Text PDFGenetic Analysis of Intraductal Carcinoma of the Prostate Detected in High-Grade Prostatic Intraepithelial Neoplasia Cases.
Cureus
December 2024
Department of Urology, Ehime University Graduate School of Medicine, Toon, JPN.
Background The accurate diagnosis of intraductal carcinoma of the prostate (IDC-P) is occasionally challenging due to the similarity in pathological morphology between IDC-P and high-grade prostatic intraepithelial neoplasia (HGPIN). In this report, we reviewed the pathology of cases previously diagnosed as HGPIN to search for IDC-P cases effectively. In addition, we examined whether those cases had genetic abnormalities.
View Article and Find Full Text PDFAssessment of Non-alcoholic Fatty Liver Disease and Level of Risk of Fibrosis in Diabetic and Non-diabetic Individuals.
Cureus
December 2024
Epidemiology and Biostatistics, Center for Medical Research and Development (CMRD), Dhaka, BGD.
Background and aim Non-alcoholic fatty liver disease (NAFLD), now known as metabolic dysfunction-associated steatotic liver disease (MASLD), is more common in people with type-2 diabetes mellitus (T2DM) than in people without diabetes mellitus (non-DM). This disease can lead to cirrhosis or hepatic cancer. There is limited data on NAFLD prevalence and the level of risk of fibrosis in Bangladeshi individuals.
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