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Assessment of Malaria Rapid Diagnostic Tests and Histidine-Rich Protein 2 Deletions among Asymptomatic Children and Adults in Bagamoyo, Tanzania.
Am J Trop Med Hyg
January 2025
Institute of Global Health and Infectious Diseases, University of North Carolina, Chapel Hill, North Carolina.
Malaria rapid diagnostic tests (mRDTs) that detect histidine-rich protein 2 (HRP2) remain the mainstay of falciparum malaria diagnosis in Sub-Saharan Africa. Understanding their test characteristics when used for surveillance in asymptomatic populations is important. We explored the rate of false-positive and false-negative mRDT results among asymptomatic persons >5 years old screened for malaria at schools and clinics in the rural Bagamoyo District using 18S ribosomal RNA real-time polymerase chain reaction (qPCR) as the reference test.
View Article and Find Full Text PDFPlasmon-Enhanced Fluorescence Paper Lateral Flow Strip for Point-of-Care Testing of SARS-CoV-2 Antigens.
Anal Chem
January 2025
Department of Chemical Engineering, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States.
Currently commercial colorimetric paper lateral flow immunoassays exhibit insufficient limit of detection (LOD) and limited clinical sensitivity toward the detection of SARS-CoV-2 antigens, which causes a high false negative rate. To mitigate this issue, a new plasmon-enhanced fluorescence probe was developed for paper lateral flow strips (PLFSs). The probe is made of a sandwich-structured Ag-core@silica@dye@silica-shell nanoparticle in which fluorescent dyes are sandwiched between the plasmonic Ag core and the silica outer shell, and the separation distance between the Ag core and the dye molecules is controlled by the silica space layer.
View Article and Find Full Text PDFChallenges in the diagnosis and follow-up of Cushing's syndrome: Evaluating LC-MS/MS as a confirmation method for urinary free cortisol analyses.
Clin Chim Acta
January 2025
Fundación Instituto de Investigación Sanitaria Islas Baleares (IdISBa), Hospital Universitario Son Espases, Carretera de Valldemossa, 79, 07010, Palma, Spain.
24-h urinary free cortisol (UFC) measurements are fundamental in the diagnosis and follow-up of Cushinǵs syndrome (CS) and immunoassays (IA) are the most widely used tests for its quantification in clinical laboratory practice. However, their suitability has been questioned mainly due to their limitations concerning analytical specificity. The aim of this research project was to evaluate a novel algorithm for CS diagnosis and follow-up in the clinical laboratory, based on the combination of IA tests with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for UFC quantification.
View Article and Find Full Text PDFUltrasensitive protein-level detection for respiratory infectious viruses.
Front Immunol
December 2024
Department of Biology, Waseda University, Tokyo, Japan.
Influenza virus, adenovirus, and respiratory syncytial virus cause major respiratory infections. These infections have similar initial symptoms making it difficult to differentiate them based on symptoms alone. PCR is currently used as the standard diagnostic test for these infections, however, it has its limitations such as non-specific and false-negative amplifications, high cost, and the inability to distinguish between a live or dead virus.
View Article and Find Full Text PDFMultiple Tolerization Subtractive Immunization in the Obtention of Specific Monoclonal Antibodies Against .
Monoclon Antib Immunodiagn Immunother
December 2024
School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, Brazil.
Paracoccidioidomycosis (PCM) is a chronic endemic mycosis in Latin America, predominantly caused by (Pb18) and (Pl01). Diagnosing PCM is challenging due to species-specific antigenic differences, therefore new biomarkers for accurate and rapid detection are needed. This study explores multiple tolerization subtractive immunization (MTSI) to generate monoclonal antibodies against rare or weakly expressed epitopes of Pb18 and Pl01, potentially improving PCM diagnosis.
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