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A convenient and economical strategy for multiple-target electrochemiluminescence detection using peroxydisulfate solution.
Talanta
January 2023
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China. Electronic address:
As various aptasensors are adopted in clinical diagnosis, the development of convenient multiple-target determination is a field of ever-increasing interests. Herein, a label-free and amplified electrochemiluminescence (ECL) sensing platform was constructed to detect multiple targets of hemin, glucose and thrombin (TB) using peroxydisulfate (SO) solution, which was one of the most convenient and economical ECL systems. It was worth mentioning that the target-induced bi-enzyme cascade catalysis reaction was developed to increase the ECL response strongly of SO solution due to the production of (O)* from the inter-reaction between reactive oxygen species (ROS) and sulfate radical (SO•-).
View Article and Find Full Text PDFVersatile electrochemical biosensor based on bi-enzyme cascade biocatalysis spatially regulated by DNA architecture.
Biosens Bioelectron
February 2021
College of Chemistry and Chemical Engineering, Research Center for Intelligent and Wearable Technology, Qingdao University, Qingdao, 266071, PR China. Electronic address:
The regulation of biocatalytic cascades in microenvironments for high performance and extended applications is still challenging. Herein, we develop a rolling circle amplification (RCA)-based one-pot method to prepare the micron-sized DNA flowers (DFs), which achieve the co-encapsulation and spatial regulation of bi-enzyme molecules, glucose oxidase (GOx) and horseradish peroxidase (HRP). In this system, GOx and HRP are integrated into the DFs simultaneously during RCA with the bridging of magnesium between enzyme residues and phosphate backbones on DFs.
View Article and Find Full Text PDFA signal amplification system constructed by bi-enzymes and bi-nanospheres for sensitive detection of norepinephrine and miRNA.
Biosens Bioelectron
January 2019
College of Materials Science and Engineering, Institute for Graphene Applied Technology Innovation, Collaborative Innovation Centre for Marine Biomass Fibers, Materials and Textiles of Shandong Province, Qingdao University, Qingdao 266071, China; College of Life Sciences, Qingdao University, Qingdao 266071, China. Electronic address:
Achieving the enhanced sensitivity and stability is always the pursuit for the fabrication of enzymatic biosensors. However, their sensitivity was still restricted by the fluctuant detection target (e.g.
View Article and Find Full Text PDFIn Situ Generation and Consumption of H2O2 by Bienzyme-Quantum Dots Bioconjugates for Improved Chemiluminescence Resonance Energy Transfer.
Anal Chem
June 2016
Analytical & Testing Center, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China.
Exploration of quantum dots (QDs) as energy acceptors revolutionizes the current chemiluminescence resonance energy transfer (CRET), since QDs possess large Stokes shifts and high luminescence efficiency. However, the strong and high concentration of oxidant (typically H2O2) needed for luminol chemiluminescence (CL) reaction could cause oxidative quenching to QDs, thereby decreasing the CRET performance. Here we proposed the use of bienzyme-QDs bioconjugate as the energy acceptor for improved CRET sensing.
View Article and Find Full Text PDFElectrochemical aptasensor for the detection of adenosine by using PdCu@MWCNTs-supported bienzymes as labels.
Biosens Bioelectron
December 2015
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address:
A highly sensitive electrochemical adenosine aptasensor was fabricated by covalently immobilizing 3'-NH2-terminated capture probe (SSDNA1) and thionine (TH) on Au-GS modified glassy carbon electrode. 3'-SH-terminated adenosine aptamer (SSDNA2) was adsorbed onto palladium/copper alloyed supported on MWCNTs (PdCu@MWCNTs)-conjugated multiple bienzymes, glucose oxidase (GOx), and horseradish peroxidase (HRP) (SSDNA2/PdCu@MWCNTs/HRP/GOx). Then, it was immobilized onto the electrode surface through the hybridization between the adenosine aptamer and the capture probe.
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