Ochratoxin production was tested in 172 strains representing species in sections Fumigati, Circumdati, Candidi, and Wentii of the genus Aspergillus by an immunochemical method using a monoclonal antibody preparation against ochratoxin A. Ochratoxin A was detected in Aspergillus ochraceus, A. alliaceus, A. sclerotiorum, A. sulphureus, A. albertensis, A. auricomus, and A. wentii strains. This is the first report of production of ochratoxins in the latter three species. Ochratoxin production by these species was confirmed by high-performance thin-layer chromatography and by high-performance liquid chromatography. The chemical methods also indicated the production of ochratoxin B by all of the Aspergillus strains mentioned above.
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http://dx.doi.org/10.1128/aem.62.12.4461-4464.1996 | DOI Listing |
Toxins (Basel)
January 2025
Multidisciplinary Agroindustry Research Laboratory, Carrera de Ingeniería en Construcción, Instituto de Ciencias Químicas Aplicadas, Universidad Autónoma de Chile, Talca 3460000, Chile.
Significant agro-industrial waste is produced during the winemaking process, including grape stalks, which are a rich source of the valuable biopolymer holocellulose that can be utilized for biotechnological processes. The purpose of this study was to delignify grape stalks in order to extract holocellulose. Then Lactobacillus plantarum (LP) was immobilized in the interstitial spaces of holocellulose and then coated with natural polymers (chitosan, Ch; and alginate, Al) to create the Holo-LP/Ch/Al complex.
View Article and Find Full Text PDFToxins (Basel)
December 2024
Laboratory of Plant Pathology, Department of Crop Science, Agricultural University of Athens, 118 55 Athens, Greece.
is considered one of the main fungi responsible for black and sour rot in grapes, as well as the production of the carcinogenic mycotoxin ochratoxin A. The global regulatory methyltransferase protein controls the production of various secondary metabolites in species, as well as influences sexual and asexual reproduction and morphology. The goal of this study was to investigate the role of the regulatory gene in physiology, virulence, and ochratoxin A (OTA) production by deleting this gene from the genome of a wild-type strain.
View Article and Find Full Text PDFFront Microbiol
January 2025
Laboratory of Food Microbiology and Mycotoxicology, Department of Food Engineering, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Brazil.
Introduction: Microbial contamination remains a vital challenge across the food production chain, particularly due to mycotoxins-secondary metabolites produced by several genera of fungi such as , and . These toxins, including aflatoxins, fumonisins, ochratoxins, and trichothecenes (nivalenol, deoxynivalenol, T2, HT-2). These contaminants pose severe risks to human and animal health, with their potential to produce a variety of different toxic effects.
View Article and Find Full Text PDFMalays J Med Sci
December 2024
First Department of Pathology, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece.
Food safety is a key priority for public health. However, consumer demand for cheese products may expose the population to the risk of mycotoxicosis and cancer, among others. Acute mycotoxicosis and cancer are examples of linked disorders.
View Article and Find Full Text PDFRaisins are so popular in the human diet as a nutritional and sweet snack. The quality of this foodstuff depends on drying conditions. To minimize ochratoxin A (OTA) content and yeast and mold content (YMC) in raisins with favorable physicochemical and sensory properties, the response surface methodology (RSM) and the face-centered central composite design (FCCD) were utilized.
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