Methods of microwave and vacuum-accelerated fixation, dehydration, and paraffin embedding are described, using a new type of vacuum and temperature stabilizable microwave histoprocessor: MFX-800. The whole histoprocessing cycle lasts 3.5-5.5 h, depending on the thickness of the tissue blocks. Well-preserved structural detail, intense staining, and good antigen preservation were achieved with various tissues. This new histoprocessing facility is recommended for routine pathology laboratories in speeding up their processing procedures.
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DNA and RNA isolated from tissues processed by microwave-accelerated apparatus MFX-800-3 are suitable for subsequent PCR and Q-RT-PCR amplification.
Pathol Oncol Res
October 2007
1st Department of Pathology and Experimental Cancer Research, Faculty of Medicine, Semmelweis University, Budapest, H-1085, Hungary.
Over the past decade, methods of molecular biology have appeared in diagnostic pathology and are routinely applied on formalin-fixed, paraffin-embedded histological samples, processed via conventional embedding methods. Due to its reagent- and cost-effectiveness, embedding techniques that utilize microwave acceleration in one or more steps of histoprocessing are increasingly used by numerous laboratories. The demand arises that tissues processed this way should also be suitable for the requirements of molecular pathology.
View Article and Find Full Text PDFOptimized quality of histologic images allows the use of neural network scanning in diagnosis of fungal infection of abnormal nails.
Anal Quant Cytol Histol
April 2006
Leiden Cytology and Pathology Laboratory, Leiden, The Netherlands.
Objective: The neural network scanning (NNS) system, formerly known as Papnet, is capable of selecting fungi in cervical smears. The objective of this study was to investigate whether the optimized quality of histologic images created using a combination of coagulant fixation and microwave histoprocessing allows the application of this computer-assisted microscopy in the diagnostic process.
Study Design: In a prospective study, 117 abnormal nails clinically suspect for fungal disease werefixed in a coagulant fixative, BoonFix, processed in a microwave histoprocessor to obtain optimal paraffin sections and stained with the periodic acid-Schiff (PAS) method.
Ultra-rapid microwave-stimulated tissue processing with a modified protocol incorporating microwave fixation.
Pathology
August 2004
Immunohistology Unit, Hunter Area Pathology Service, and Discipline of Anatomical Pathology, University of Newcastle, Newcastle NSW 2310, Australia.
Aims: To develop an ultra-rapid microwave (MW)-stimulated histoprocessing protocol that incorporates MW fixation and produces consistent, high quality sections.
Methods: A range of fresh autopsy tissues was divided into three groups composed of equal numbers of small and large tissue blocks. Group 1 tissues were fixed for 8 hours in 4% buffered formaldehyde and processed in a conventional tissue processor through a 15-hour cycle.
Incorporation of microwave tissue processing into a routine pathology laboratory: impact on turnaround times and laboratory work patterns.
Pathology
August 2004
Division of Anatomical Pathology, Hunter Area Pathology Service, Newcastle, NSW 2310, Australia.
Aims: To examine the impact of microwave (MW) tissue processing on turnaround times (TATs) in a routine diagnostic laboratory.
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Results And Conclusions: The TAT for conventional tissue processing was almost 21 h compared with 6.
[A method of preparing sections for express histological diagnosis].
Arkh Patol
October 2000
Cancer Research Institute, Rostov/Don.
The method of improving quality of histological slides for intrasurgical diagnosis is proposed. The method consists in preliminary microwave processing of the slice of surgical material before putting in into cryostat for freezing and sections preparation. A slice of surgical material was first immersed in 10 ml of physiological solution (pH = 7.
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