An improved method for embedding specimens in polyethylene glycol (PEG) 4000, a water soluble polymer, was used to prepare the vestibular end-organs of the inner ear. Staining of the tissue sections of PEG embedded specimens with antibodies to alpha-tubulin and to calmodulin was compared with staining of tissue sections of Araldite and Unicryl embedded specimens. PEG embedded sections revealed sensitive immunocytochemical labeling with excellent morphological resolution. The problems of embedding and orienting small specimens of the inner ear in PEG are described and the methods used to solve them are described.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3950/jibiinkoka.99.361 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
3D printed dummy heads for crosstalk cancellation studies in bone conduction.
HardwareX
March 2025
Center for Frontier Medical Engineering, Chiba University, Chiba, Japan.
Thanks to affordable 3D printers, creating complex designs like anatomically accurate dummy heads is now accessible. This study introduces dummy heads with 3D-printed skulls and silicone skins to explore crosstalk cancellation in bone conduction (BC). Crosstalk occurs when BC sounds from a transducer on one side of the head reach the cochlea on the opposite side.
View Article and Find Full Text PDFA rare haplotype of the GJD3 gene segregating in familial Meniere's disease interferes with connexin assembly.
Genome Med
January 2025
Otology & Neurotology Group CTS495, Instituto de Investigación Biosanitario, Ibs.GRANADA, Universidad de Granada, 18071, Granada, Spain.
Background: Familial Meniere's disease (FMD) is a rare polygenic disorder of the inner ear. Mutations in the connexin gene family, which encodes gap junction proteins, can also cause hearing loss, but their role in FMD is largely unknown.
Methods: We retrieved exome sequencing data from 94 individuals in 70 Meniere's disease (MD) families.
A Neuron-Like Cellular Model for Severe Tinnitus Associated with Rare Variations in the ANK2 Gene.
Mol Neurobiol
January 2025
Otology & Neurotology Group CTS495, Division of Otolaryngology, Department of Surgery, Instituto de Investigación Biosanitaria, Ibs.GRANADA, Granada, Universidad de Granada, Granada, Spain.
Tinnitus is the perception of sound without an external source, often associated with changes in the auditory pathway and different brain regions. Recent research revealed an overload of missense variants in the ANK2 gene in individuals with severe tinnitus. ANK2, encoding ankyrin-B, regulates axon branching and inhibits microtubule invasion.
View Article and Find Full Text PDFVestibular stimulation and space-time interaction affect the perception of time during whole-body rotations.
PLoS One
January 2025
COMETE U1075, Inserm, CYCERON, Université de Caen Normandie, Caen, France.
Among the factors, such as emotions, that distort time perception, vestibular stimulation causes a contraction in subjective time. Unlike emotions, the intensity of vestibular stimulation can be easily and precisely modified, making it possible to study the quantitative relationship between stimulation and its effect on time perception. We hypothesized that the contraction of subjective time would increase with the vestibular stimulation magnitude.
View Article and Find Full Text PDFMetabolome modification and underlying biomarker of noise-induced hearing loss Guinea pig cochlear fluid.
Acta Otolaryngol
January 2025
Department of Otolaryngology Head and Neck Surgery, the First Medical Center of Chinese PLA General Hospital, Beijing, China.
Background: Noise-induced hearing loss (NIHL) is a kind of acquired sensorineural hearing loss and has shown an increasing incidence in recent years. Hence, elucidating the exact pathophysiological mechanisms and proposing effective treatment and prevention methods become the top priority. Though a great number of researches have been carried out on NIHL, few of them were focused on metabolites.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!