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qPCR as a Tool for the Diagnosis of Visceral and Cutaneous Leishmaniasis: A Systematic Review and Meta-Analysis.
Acta Parasitol
January 2025
Ezequiel Dias Foundation, Directorate of Research and Development, Belo Horizonte, Minas Gerais, 30510-010, Brazil.
Introduction: Ensuring accuracy in the diagnosis of leishmaniasis is crucial due to the myriad of potential differential diagnoses. Given the inherent limitations of serological techniques, real-time polymerase chain reaction (qPCR) emerges as a superior alternative. Furthermore, parasitological methods, conventionally regarded as the gold standard owing to their high specificity, encounter challenges concerning sensitivity and invasiveness for patients.
View Article and Find Full Text PDFLiver parasites: A global endemic and journey from infestation to intervention.
World J Gastroenterol
January 2025
Department of Medicine, Section of Gastroenterology, Aga Khan University Hospital, Karachi 75500, Sindh, Pakistan.
Parasites have coexisted with humans throughout history, forming either symbiotic relationships or causing significant morbidity and mortality. The liver is particularly vulnerable to parasitic infections, which can reside in, pass through, or be transported to the liver, leading to severe damage. This editorial explores various parasites that infect the liver, their clinical implications, and diagnostic considerations, as discussed in the article "Parasites of the liver: A global problem?".
View Article and Find Full Text PDFValidation of an improved reference freeze-dried direct agglutination test for detecting leishmaniasis in the canine reservoir.
Access Microbiol
January 2025
Department of Medical Laboratory Science, Faculty of Medical and Health Sciences, Liwa College, Abu Dhabi, UAE.
Proper identification and management of post-kala-azar dermal leishmaniasis (PKDL) and canine leishmaniasis (CanL) cases are among the prerequisites to the effective control of visceral leishmaniasis worldwide. Unlike PKDL, CanL still awaits effective improvement because of its cryptic nature, absence of parasites in lesions or lymph nodes and not complete sensitivity of some diagnostic tools in use. Because of the need for certain skills and equipment, both the liquid direct agglutination test and freeze-dried direct agglutination test (FD-DAT) versions are, in comparison with the indirect immunofluorescence antibody test (IFAT) or enzyme-linked immunosorbent assay (ELISA), practical and feasible diagnostic alternatives.
View Article and Find Full Text PDFLeishmaniasis in Patients With Inflammatory Bowel Disease: A National Multicenter Study of GETECCU.
United European Gastroenterol J
January 2025
Gastroenterology Department, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Hospital General Universitario Dr Balmis de Alicante, Instituto de Investigación Sanitaria y Biomédica de Alicante (ISABIAL), Madrid, Spain.
Background: Leishmaniasis (LI) is a vector-borne illness caused by a protozoan of the genus Leishmania. Data on the features of LI in patients with inflammatory bowel disease (IBD) are scarce.
Aim: To describe the characteristics of patients with IBD who present with leishmaniasis, infection outcomes and the risk factors associated with developing visceral leishmaniasis (VL).
Rapid isothermal molecular tests to discriminate between Leishmania braziliensis and Leishmania infantum infections in dogs.
Parasit Vectors
January 2025
Aggeu Magalhães Institute, Oswaldo Cruz Foundation (Fiocruz), Recife, Brazil.
Background: We standardized two recombinase polymerase amplification (RPA) assays coupled with lateral flow (LF) strips for the detection of Leishmania braziliensis and Leishmania infantum kinetoplast DNA (kDNA).
Methods: The RPA-LF assays were tested at different temperatures and reaction times, using DNA from cultured L. braziliensis and L.
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