AI Article Synopsis
- The microsomal delta 12-desaturase system converts oleic acid into linoleic acid with a specific activity of approximately 16 pmol/min/mg protein, but most of its activity remains nonsedimentable even after extensive centrifugation.
- The enzymatic complex functions best at a pH between 7 and 8 and exhibits low thermal stability, with Ca2+ and Hg2+ being strong inhibitors while Mg2+, Mn2+, and Zn2+ act as activators.
- The system shows a preference for oleic acid and responds differentially to its isomers, with oleoyl-CoA being favored over elaidoyl-CoA in stimulating activity.
Article Abstract
The specific activity of the microsomal delta 12-desaturase system, which transforms oleic acid into linoleic acid, was about 16 pmol/min/mg protein. However, most of the total activity was nonsedimentable even after a 200000 x g centrifugation for 100 min. The study of various physicochemical parameters showed that this enzymatic complex, functioning optimally between pH 7 and 8, had low thermal stability. Ca2+ which may cause an aggregation of the microsomes, and Hg2+ completely inhibited the activity, whereas Mg2+, Mn2+, and Zn2+ were activators. The delta 12-desaturase system was relatively specific toward oleic acid, though isomers of this fatty acid also had an action, either as substrates or as competitive inhibitors, on the activity of the system. The study of the effect of the exogenous oleoyl-CoA and elaidoyl-CoA on the specific activity of the delta 12-desaturase system showed a preference toward oleoyl-CoA.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF02529871 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
In Silico Identification and Characterization of Fatty Acid Desaturase () Genes in L. Skeels: Implications for Oil Quality and Abiotic Stress.
Bioinform Biol Insights
May 2024
Team of Microbiology and Molecular Biology, Plant and Microbial Biotechnology, Biodiversity and Environment Research Center, Faculty of Sciences, Mohammed V University, Rabat, Morocco.
Fatty acid desaturase () is the key enzyme that leads to the formation of unsaturated fatty acids by introducing double bonds into hydrocarbon chains, and it plays a critical role in plant lipid metabolism. However, no data are available on enzyme-associated genes in argan trees. In addition, a candidate gene approach was adopted to identify and characterize the gene sequences of interest that are potentially involved in oil quality and abiotic stress.
View Article and Find Full Text PDFMorphological Changes and Strong Cytotoxicity in by Overexpressing Delta-12-Desaturase.
J Fungi (Basel)
February 2024
Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, China.
In this study, delta-12 desaturase was overexpressed in using the single-copy integrative vector pINA1312 and multicopy integrative vector pINA1292, resulting in the engineered yeast strains 1312-12 and 1292-12, respectively. The content of intracellular linoleic acid (LA) in the 1292-12 strain was much higher than in the 1312-12 strain and the control group. One interesting finding was that the 1292-12 strain showed obvious changes in surface morphology.
View Article and Find Full Text PDFHomologous and Heterologous Expression of Delta(12)-Desaturase in Enhanced the Production of Linolenic Acid.
Molecules
August 2022
Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, China.
Linolenic acid (LA) is gaining more interest within the scientific community. This is because it has a potential medical role in reducing the risk of inflammation, carcinogenesis, atherosclerosis and diabetes and is a valuable nutraceutical for human health. The oleaginous fungus produces a high lipid content (36%), including valuable polyunsaturated fatty acids (PUFAs).
View Article and Find Full Text PDFPolyunsaturated fatty acids promote the rapid fusion of lipid droplets in Caenorhabditis elegans.
J Biol Chem
August 2022
Center for Life Sciences, School of Life Sciences, State Key Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan, China. Electronic address:
Lipid droplets (LDs) are intracellular organelles that dynamically regulate lipids and energy homeostasis in the cell. LDs can grow through either local lipid synthesis or LD fusion. However, how lipids involving in LD fusion for LD growth is largely unknown.
View Article and Find Full Text PDFA non-canonical Δ9-desaturase synthesizing palmitoleic acid identified in the thraustochytrid Aurantiochytrium sp. T66.
Appl Microbiol Biotechnol
August 2021
Department of Biotechnology and Food Science, NTNU Norwegian University of Science and Technology, Trondheim, Norway.
Thraustochytrids are oleaginous marine eukaryotic microbes currently used to produce the essential omega-3 fatty acid docosahexaenoic acid (DHA, C22:6 n-3). To improve the production of this essential fatty acid by strain engineering, it is important to deeply understand how thraustochytrids synthesize fatty acids. While DHA is synthesized by a dedicated enzyme complex, other fatty acids are probably synthesized by the fatty acid synthase, followed by desaturases and elongases.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!