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Identification and characterization of substrate- and product-selective nylon hydrolases.
bioRxiv
November 2024
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA 37830.
Article Synopsis
- - The study investigated a diverse group of 95 enzymes from the N-terminal nucleophile hydrolase superfamily, focusing on their ability to hydrolyze synthetic polyamides like nylon.
- - About 40% of these enzymes demonstrated significant nylon hydrolase activity, comparable to the well-known enzyme NylC, without any clear link between their evolutionary relationships and their hydrolytic capabilities.
- - Researchers identified several enzymes with strong substrate selectivity, especially those that efficiently processed Nylon 6,6, and also analyzed the crystal structure of a specific hydrolase to better understand the factors affecting its activity and selectivity.
Comparative performance analysis of Synovasure™ and Leukocyte Esterase assays for the diagnosis of periprosthetic infections in complex microbiological situations.
Orthop Traumatol Surg Res
November 2024
Centre de Référence Pour le Traitement des Infections Ostéo-Articulaires Complexes (CRIOAC), Avenue du Professeur-Emile-Laine, 59037 Lille-Tourcoing, France; Université Lille-Hauts de France, 59000 Lille, France; Service Universitaire des Maladies Infectieuses et du Voyageur, Hôpital Dron, 59200, Tourcoing, France.
Introduction: Osteoarticular infections (OAI) after prosthetic surgery have serious functional and economic consequences. Rapid tests for alpha-defensin (TAD) and leukocyte esterase (TLE) are two intra-articular markers involved in the diagnosis of OAIs. TLE cannot be applied in the presence of blood unless centrifugation is used, but the rate of "non-application" of the test for this reason is unknown in complex microbiological situations (discordant or negative puncture, ongoing antibiotic treatment).
View Article and Find Full Text PDFQuantifying platinum binding on protein-functionalized magnetic microparticles using single particle-ICP-TOF-MS.
Anal Methods
May 2024
Radioisotope Science and Technology Division, Oak Ridge National Laboratory Oak Ridge, TN, USA.
This work describes an analytical procedure, single particle-inductively coupled plasma-time-of-flight-mass spectrometry (SP-ICP-TOF-MS), that was developed to determine the platinum binding efficiency of protein-coated magnetic microparticles. SP-ICP-TOF-MS is advantageous due to its ability to quasi-simultaneously detect all nuclides (Li-Pu), allowing for both platinum and iron (composition of magnetic microparticles) to be measured concurrently. This method subsequently allows for the differentiation between bound and unbound platinum.
View Article and Find Full Text PDFEffects of portable pedal machines at work on lipoprotein subfraction profile in sedentary workers - the REMOVE study.
Lipids Health Dis
April 2024
INRAE, UNH, CHU Clermont-Ferrand, Department of Sport Medicine and Functional Exploration, Université Clermont Auvergne, Clermont-Ferrand, France.
Background: Sedentary behaviour at work is a major cause of atherosclerosis, particularly in tertiary workers. However, no studies have ever assessed the effect of active workstation on lipoprotein subfraction profile. This study aimed to evaluate the effect of 12-week portable pedal machines (PPMs) on lipoprotein subfraction profile among healthy sedentary workers.
View Article and Find Full Text PDFValidation of a metabolite-GWAS network for family 1 UDP-glycosyltransferases.
Front Plant Sci
July 2023
Center for Bioenergy Innovation, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.
Metabolite genome-wide association studies (mGWASs) are increasingly used to discover the genetic basis of target phenotypes in plants such as , a biofuel feedstock and model woody plant species. Despite their growing importance in plant genetics and metabolomics, few mGWASs are experimentally validated. Here, we present a functional genomics workflow for validating mGWAS-predicted enzyme-substrate relationships.
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