The formaldehyde resistance mechanisms in the formaldehyde-resistant strain Escherichia coli VU3695 were investigated. A large (4.6-kb) plasmid DNA fragment encompassing the formaldehyde resistance gene was sequenced. A single 1,107-bp open reading frame encoding a glutathione- and NAD-dependent formaldehyde dehydrogenase was identified and sequenced, and the enzyme was expressed in an in vitro assay and purified. Amino acid sequence homology studies showed 62.4 to 63.2% identity with class III alcohol dehydrogenases isolated from horse, human, and rat livers. We demonstrated that the resistance mechanism in the formaldehyde-resistant strain E. coli VU3695 and in other formaldehyde-resistant members of the family Enterobacteriaceae is based on the enzymatic degradation of formaldehyde by a formaldehyde dehydrogenase.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC163518 | PMC |
| http://dx.doi.org/10.1128/AAC.40.10.2276 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Synthesis of an exfoliated kaolinite-poly(urea-formaldehyde) nanocomposite.
RSC Adv
January 2025
Laboratory of Applied Inorganic Chemistry, Department of Inorganic Chemistry, University of Yaoundé I P.O. Box 812 Yaoundé Cameroon
In this study, kaolinite-poly(urea-formaldehyde) was successfully prepared through the polymerization of urea intercalated within the kaolinite structure. Polymerization was carried out under ambient conditions by immersing kaolinite-urea in formaldehyde. Evidence of urea intercalation and polymerization was obtained from FTIR, XRD, and thermal analysis (TG-DSC).
View Article and Find Full Text PDFFabrication and Performance Enhancement of Wood Liquefaction-Based Carbon Fibers Modified with Alumina Nanoparticles.
Polymers (Basel)
January 2025
College of Resources, Environment and Materials, Guangxi University, Nanning 530004, China.
In this paper, alumina-modified wood liquefaction (AL-WP) was prepared by blending nano-alumina (AlO) into wood liquefaction phenolic resin (WP) using a co-blending method. Alumina-modified wood liquefaction protofilament fiber (AL-WPF) was obtained by melt-spinning, curing, and thermo-curing processes, which were followed by carbonization to obtain alumina-modified wood liquefaction carbon fiber (AL-WCF). This paper focuses on the enhancement effect of nano-alumina doping on the mechanical properties and heat resistance of wood liquefaction carbon fiber (WCF), explores the evolution of graphite microcrystalline structure during the high-temperature carbonization process, and optimizes the curing conditions of AL-WPF.
View Article and Find Full Text PDFCr-Doped Nanocrystalline TiO-CrO Nanocomposites with p-p Heterojunction as a Stable Gas-Sensitive Material.
Int J Mol Sci
January 2025
Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia.
Nanocrystalline TiO is a perspective semiconductor gas-sensing material due to its long-term stability of performance, but it is limited in application because of high electrical resistance. In this paper, a gas-sensing nanocomposite material with p-p heterojunction is introduced based on p-conducting Cr-doped TiO in combination with p-conducting CrO. Materials were synthesized via a single-step flame spray pyrolysis (FSP) technique and comprehensively studied by X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) specific surface area analysis, transition electron microscopy (TEM), energy dispersive X-ray (EDX) spectroscopy, X-ray photoelectron spectroscopy (XPS), electron paramagnetic resonance (EPR), and Raman spectroscopy.
View Article and Find Full Text PDFOxygen vacancy and interface effect dual modulation of SnS/SnO heterojunction for boosting formaldehyde detection at low temperature.
Talanta
January 2025
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, PR China. Electronic address:
Formaldehyde (HCHO) is a harmful volatile organic pollutant, which is commonly found in interior decoration and furniture products. Therefore, it is necessary to develop a gas sensor that can quickly and accurately detect formaldehyde for human health and environmental protection. In order to achieve this goal, in this work, SnS/SnO heterostructure was synthesized by in-situ sulfurization process on the basis of SnO nanospheres, and its formaldehyde sensing performance was studied.
View Article and Find Full Text PDFEvaluation of Four Methods to Determine the Degree of Cure of Melamine-Based Direct Pressed Laminates on Particleboards: Two Improved UV Absorption Methods, the Kiton Test, and Near Infrared Spectroscopy.
Materials (Basel)
December 2024
Fraunhofer WKI, 38108 Braunschweig, Germany.
Despite its importance, the determination of the degree of cure of melamine-based laminates often relies on tests with limited accuracy and validity. Undercured surfaces may suffer insufficient resistance to scratching and heat as well as substandard surface quality. Overcured melamine surfaces tend to crack and entail the inefficient utilization of the press-the panels could have been pressed for a shorter time.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!