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Comparative Proteomics of Resistant and Susceptible Strains of Frankliniella occidentalis to Abamectin.
Electrophoresis
January 2025
Department of Cell & Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae) is an invasive agricultural pest with developed resistance to abamectin in some strains due to frequent treatment with the pesticide. In this study, we examined differentially expressed proteins (DEPs) between abamectin-resistant (Aba; under abamectin selective pressure) and susceptible strains (Aba; without abamectin selective pressure) of F. occidentalis.
View Article and Find Full Text PDFCell confinement by micropatterning induces phenotypic changes in cancer-associated fibroblasts.
Acta Biomater
January 2025
Translational Tumor Engineering Laboratory, Department of Biomedical Engineering, National University of Singapore, Singapore 119276, Singapore; Cancer Science Institute, National University of Singapore, Singapore 117599, Singapore; The N.1 Institute for Health, National University of Singapore, Singapore 117456, Singapore. Electronic address:
Article Synopsis
- * The research explored how the shape and confinement of CAFs on specially designed surfaces influence their morphology and gene expression, finding that certain conditions led to changes toward more inflammatory and desmoplastic CAF types.
- * The findings suggest that the way CAFs are physically arranged affects their response to cancer therapies, suggesting that understanding CAF plasticity can help develop better treatment strategies.
Key Proteins in Rat Cerebral Cortex: Application of Extract as a Neuroprotective Agent in Alzheimer's Type Dementia.
Molecules
November 2024
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl. 9, 1113 Sofia, Bulgaria.
Alzheimer's disease (AD) is the most widespread neurodegenerative disorder. Recently, it was found that mucus extract from has beneficial effects on memory and cognitive processes in a rat scopolamine model of AD. The present study elucidated the mechanisms of action of standardized mucus snail extract (SE) enriched with a fraction above 20 kDa on Alzheimer-type dementia in rats.
View Article and Find Full Text PDFProtein profile changes during priming explants to embryogenic response in : identification of the RPN12 proteasome subunit involved in the protein degradation.
PeerJ
November 2024
Unidad de Biología Integrativa, Centro de Investigacion Cientifica de Yucatan (CICY), Mérida, Yucatán, Mexico.
Plant somatic embryogenesis encompasses somatic cells switch into embryogenic cells that can later produce somatic embryos with the ability to produce plantlets. Previously, we defined culture settings for the somatic embryogenesis process of that comprise adequate plantlets with auxin plus cytokinin followed by cut-leaf explant cultivation with cytokinin, producing embryos with the ability to regenerate plantlets. Here, we confirmed that cultivating cut-leaf explants with cytokinin is sufficient to promote somatic embryos proliferation and the high yield of somatic embryos in the protocol requires adequate plantlets with auxin plus cytokinin.
View Article and Find Full Text PDFUnveiling Histone Proteoforms using 2D-TAU Gel Electrophoresis.
J Vis Exp
October 2024
Research Center on Advanced Biochemistry and Molecular Biology, Department of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro;
Article Synopsis
- Histones undergo various post-translational modifications (PTMs) that affect nucleosome dynamics and cell fate, with histone PTM regulation being essential for normal cellular function.
- The method described combines two types of gel electrophoresis—TAU-GEL and SDS-PAGE—to effectively isolate and analyze histone isoforms, providing a detailed two-dimensional map.
- This innovative proteomic technique enhances the ability to enrich specific histone isoforms and characterize new PTMs, aiding in the understanding of abnormal cellular behaviors linked to histone deregulation.
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