RNAs, isolated from rat sceletal muscles by heat and cold phenol treatment within various periods after animal death, were analyzed by means of centrifugation in sucrose gradient. Content of 28 S RNA was found to be decreased by 7-8% within 3 days of autolysis with maintaining of the total ribosomal RNA yield. These data suggest that nucleases were present in microsomal and crude myofibrillar fractions and their activity was increased duing autolysis.
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Sci Justice
January 2025
Department of Forensic Science, School of Life Science, Atlantic Technology University (ATU), Sligo, F91 YW50, Ireland; Department of Forensic and Crime Science, Staffordshire University, College Road, Stoke-on-Trent, Staffordshire ST4 2DE, UK.
This study unveils the establishment of the United Kingdom-Netherlands Decomposition Experimental Research (UNDER) working group, marking a pioneering initiative in practical Forensic Taphonomy within the UK. Our primary objective was to craft a cohesive multidisciplinary framework, designed to ethically orchestrate, execute, and assess human decomposition. Concurrently, we aimed to amass data through human burials, fostering collaboration among diverse forensic experts across Europe.
View Article and Find Full Text PDFEnviron Microbiol
January 2025
Department of Biochemistry, Genetics and Microbiology, Centre for Microbial Ecology and Genomics, University of Pretoria, Pretoria, South Africa.
Plant detritus is abundant in grasslands but decomposes slowly and is relatively nutrient-poor, whereas animal carcasses are labile and nutrient-rich. Recent studies have demonstrated that labile nutrients from carcasses can significantly alter the long-term soil microbial function at an ecosystem scale. However, there is a paucity of knowledge on the functional and structural response and temporal scale of soil microbiomes beneath large herbivore carcasses.
View Article and Find Full Text PDFCommun Biol
January 2025
Cardio-Thoracic Translational Medicine (CTTM) Lab, Department of Surgical Sciences, Uppsala University, Uppsala, Sweden.
Recent developments in mass spectrometry-based proteomics have established it as a robust tool for system-wide analyses essential for pathophysiological research. While post-mortem samples are a critical source for these studies, our understanding of how body decomposition influences the proteome remains limited. Here, we have revisited published data and conducted a clinically relevant time-course experiment in mice, revealing organ-specific proteome regulation after death, with only a fraction of these changes linked to protein autolysis.
View Article and Find Full Text PDFSci Rep
December 2024
School of Health and Life Sciences, Teesside University, Middlesbrough, UK.
Outdoor microcosms, metabarcoding with next-generation sequencing of the 16S rRNA bacterial gene, total body score (TBS) and physicochemical analyses were used to monitor Mus musculus decomposition aboveground (A) and in the subsurface (S), and compared to soil-only controls (C). As determined by MaAsLin2 analysis, significant shifts in bacterial communities at 30 cm depths within the A, S and C treatments distinguished control from experimental soils, and between aboveground and subsurface deposition, demonstrating the potential for gravesoil discrimination during the first 90 days. For example, Dokdonella (p = 0.
View Article and Find Full Text PDFInt J Legal Med
December 2024
Department of Medical Biology, Section Clinical Anatomy and Embryology, AmsterdamUMC, Location Academic Medical Centre, Meibergdreef 9, Amsterdam, AZ, 1105, The Netherlands.
Forensic taphonomy is the study of postmortem changes of human remains for the purpose of answering legal investigative questions. Many variables can affect the pattern and rate of decomposition of remains, posing challenges for taphonomic studies and estimation of the postmortem interval. Given the gap in knowledge regarding the suitability of using frozen remains to extrapolate conclusions to fresh material, investigating the effects of freeze-thaw cycles followed by burial on human remains is vital for forensic practice and taphonomic research.
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