AI Article Synopsis
- The study aimed to investigate the effects of haemodialysis using two different membrane materials (cuprophane and polyacrylonitrile) on the release of specific leukotrienes (LT) and the roles of related metabolites in membrane incompatibility.
- Results showed that cuprophane membranes caused a significant increase in the proinflammatory leukotrienes LTC4 and LTB4, along with decreased white blood cell count and blood oxygen levels, whereas polyacrylonitrile membranes did not show these negative effects.
- The findings suggest that the release of leukotrienes during dialysis with cuprophane membranes could serve as an important indicator of membrane bioincompatibility, highlighting a potential benefit of using
Article Abstract
Unlabelled: The aim of our study was (1) to verify whether haemodialysis (HD) with cuprophane (CUP) and polyacrylonitrile (PAN) membranes is associated with the release of vasoactive leukotriene (LT) C4 and chemotactic LTB4 and (2) to analyse the respective roles of lipoxygenase and cyclo-oxygenase metabolites of arachidonic acid in membrane bio-incompatibility. The investigation was performed in 10 uremic patients using hollow-fibre dialysers and dialysed successively, in random order, with CUP and PAN membranes. The arterial and venous (from dialyser) blood was sampled for the measurement of biochemical parameters, plasma LTC4, LTB4 and prostaglandins (PG) 6-keto-F1 alpha, E2, F2 alpha and thromboxane B2 before and after 15, 30 and 240 min of HD. Eicosanoids were measured by RIA after prior extraction and HPLC separation.
Results: CUP HD was associated with a marked early leukopenia and a delayed decrease in blood pO2. Simultaneously, plasma LTB4 and LTC4 increased significantly in arterial blood after 30 min of HD and in venous blood at the end of session of 240 min. Cyclo-oxygenase metabolites increased as well, but nonsignificantly, with a maximum at the end of HD. PAN HD did not significantly change white blood cell count, pO2, or plasma eicosanoid levels.
Conclusion: CUP membranes stimulate the release of proinflammatory and vasoactive LTB4 and LTC4. PAN membrane haemodialysis is without such side effects. The release of LTs may be an additional valuable marker of membrane bioincompatibility.
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| http://dx.doi.org/10.1159/000189275 | DOI Listing |
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