The techniques of differential scanning calorimetry, fluorescence of merocyanine 540, fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene, proton permeability, and lipid peroxidation are used to compare the perturbations of cholesterol and alpha-tocopherol on lipid bilayer membranes composed of different phosphatidylcholines containing stearic acid in the sn-1 position and an unsaturated fatty acid (either oleic, alpha-linolenic, gamma-linolenic, or docosahexaenoic acid) in the sn-2 position. It is concluded that the structural roles of cholesterol and alpha-tocopherol may be similar with membranes composed of some phosphatidylcholines but are clearly different with membranes composed of other related phosphatidylcholines. alpha-Tocopherol exerts a much larger effect than cholesterol on membranes rich in polyunsaturated fatty acids that have their initial double bond before the delta 9 position. Cholesterol interacts more favorably with fatty acids that do not have an double bond before the delta 9 position. The membrane structural effects are explained in terms of the larger size of the sterol ring structure of cholesterol compared to the smaller chromanol ring of the alpha-tocopherol.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/bi961058m | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Structural characteristics of a polysaccharide isolated from Potentilla anserina L. and its mitigating effect on Zearalenone-induced oxidative damage in Sertoli cells.
Int J Biol Macromol
January 2025
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730030, China; Gansu Innovation Research Center for Swine Production Engineering and Technology, Lanzhou 730070, China. Electronic address:
The present study aims to characterize the structural features of a natural polysaccharide called PAP-1b extracted from the roots of Potentilla anserina L. and to evaluate its antioxidant activity. Structural characterization indicated that PAP-1b with a molecular weight of 1.
View Article and Find Full Text PDFA Streamlined Approach to Anticounterfeiting Technologies: Patterned AAO Membranes Based on Photonic Crystal Effects with Tunable Color Shifts and pH Responsiveness.
Small
January 2025
Department of Applied Chemistry, National Yang Ming Chiao Tung University, Hsinchu, 300093, Taiwan.
Anticounterfeiting technologies have become increasingly crucial due to the growing issue of counterfeit goods, particularly in high-value industries. Traditional methods such as barcodes and holograms are prone to replication, prompting the need for advanced, cost-effective, and efficient solutions. In this work, a practical application of anodic aluminum oxide (AAO) membranes are presented for anticounterfeiting, which addresses the challenges of high production costs and complex fabrication processes.
View Article and Find Full Text PDFInsights into the Mechanism of Protein Loading by Chain-Length Asymmetric Complex Coacervates.
Biomacromolecules
January 2025
Instituto de Química de los Materiales, Ambiente y Energía, CONICET, Universidad de Buenos Aires, Intendente Güiraldes 2160, CABA (Buenos Aires) 1428, Argentina.
The study of the phase behavior of polyelectrolyte complex coacervates has attracted significant attention in recent years due to their potential use as membrane-less organelles, microreactors, and drug delivery platforms. In this work, we investigate the mechanism of protein loading in chain-length asymmetric complex coacervates composed of a polyelectrolyte and an oppositely charged multivalent ion. Unlike the symmetric case (polycation + polyanion), we show that protein loading is highly selective based on the protein's net charge: only proteins with charges opposite to the polyelectrolyte can be loaded.
View Article and Find Full Text PDFPreparation of a CNF porous membrane and synthesis of silver nanoparticles (AgNPs).
RSC Adv
January 2025
The Center for Chemical Biology, School of Fundamental Science and Technology, Graduate School of Science and Technology, Keio University 3-14-1 Hiyoshi, Kohoku-ku Yokohama 223-8522 Japan +81-45-566-1580 +81-45-566-1839.
We prepared a cellulose nanofiber (CNF)-based porous membrane with three dimensional cellular structures. CNF was concentrated a surfactant-induced assembly by mixing CNF with a cationic surfactant, domiphen bromide (DB). Furthermore, they were accumulated by centrifugation to obtain a CNF-DB sol.
View Article and Find Full Text PDFCoacervate vesicles assembled by liquid-liquid phase separation improve delivery of biopharmaceuticals.
Nat Chem
January 2025
State Key Laboratory of Advanced Drug Delivery and Release Systems, Liangzhu Laboratory, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
Vesicles play critical roles in cellular materials storage and signal transportation, even in the formation of organelles and cells. Natural vesicles are composed of a lipid layer that forms a membrane for the enclosure of substances inside. Here we report a coacervate vesicle formed by the liquid-liquid phase separation of cholesterol-modified DNA and histones.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!