An experiment was conducted to investigate the effect of key stiffness on the development of fatigue, keyboard reaction forces, and muscle electromyography (EMG) responses. Six subjects typed continuously for 2 hours on each of two keyboards (0.28 N or 0.83 N resistance keys, presented in random order). Keyboard reaction force and root mean square finger flexor and extensor EMG were recorded for 2 minutes at 250 Hz for every 10 minutes subjects typed. After typing for 2 hours subjects were given a 2-hour rest break and then typed on the remaining keyboard for an additional 2 hours Fifty-four percent more peak force, 34% more peak finger flexor EMG, and 2% more peak finger extensor EMG were exerted while using the 0.83 N keyboard. Peak and 90th percentile values showed similar trends and were well correlated for force and finger flexor and extensor EMG. Subjects typed much harder than necessary (4.1 to 7.0 times harder on the 0.28 N keyboard and 2.2 to 3.5 times harder on the 0.83 N keyboard) to activate the keys. Fatigue was observed on the 0.83 N keyboard during 2 hours of continuous typing, but the trends were mild. It appears that the ratio of typing force to flexor EMG may not be a sensitive enough indicator of fatigue for low-force high repetition work.
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http://dx.doi.org/10.1080/15428119691014549 | DOI Listing |
Breast Cancer Res
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Department of Cell, Developmental & Cancer Biology, Oregon Health & Science University, Portland, OR, 97239, USA.
Tumor-infiltrating lymphocytes are considered clinically beneficial in breast cancer, but the significance of natural killer (NK) cells is less well characterized. As increasing evidence has demonstrated that the spatial organization of immune cells in tumor microenvironments is a significant parameter for impacting disease progression as well as therapeutic responses, an improved understanding of tumor-infiltrating NK cells and their location within tumor contextures is needed to improve the design of effective NK cell-based therapies. In this study, we developed a multiplex immunohistochemistry (mIHC) antibody panel designed to quantitatively interrogate leukocyte lineages, focusing on NK cells and their phenotypes, in two independent breast cancer patient cohorts (n = 26 and n = 30).
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