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Comprehensive characterization of tobacco-induced changes in enamel surface topography.
J Oral Biol Craniofac Res
December 2024
Department of Oral Biology and Oral Pathology, Saveetha Dental College and Hopsitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, 600077, India.
Introduction: Enamel translucency, essential for the aesthetic appeal of teeth, is primarily determined by its thickness, quality, and refractive index. Several factors, including age, genetics, diet, oral hygiene practices, fluoride exposure, and acidic challenges, can influence enamel translucency. Tobacco use, in particular, leads to significant alterations in enamel appearance by penetrating its micropores, causing yellowing and browning.
View Article and Find Full Text PDFDevelopment of a sequential laser microdissection tissue cuts workflow for the spatial and quantitative analysis of drugs in fresh frozen tissue sections.
PLoS One
December 2024
Faculty of Health, Maastricht MultiModal Molecular Imaging Institute (M4I), Medicine and Life Sciences, Maastricht University, Maastricht, The Netherlands.
Mass spectrometry imaging (MSI) is a well-established technique that allows to determine the distribution of small molecules, such as lipids, metabolites, and drugs, as well as large molecules in tissue sections. Because of the tissue heterogeneity, resulting in different matrix effects, and to the fact that the measured compounds are not entirely "extracted" from the tissue during the measurement, the absolute quantitative aspect of MSI is limited. To combine compound quantification with spatial information on fresh frozen unstained tissue sections, laser (capture) microdissection has been used to isolate tissue sections for compound extraction and LC-MS/MS quantification.
View Article and Find Full Text PDFQuantitative structural organization of the sclera in chicks after deprivation myopia measured with second harmonic generation microscopy.
Front Med (Lausanne)
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Section of Neurobiology of the Eye, Institute for Ophthalmic Research, Tuebingen, Germany.
Visual deprivation causes enhanced eye growth and the development of myopia, which is associated with a change in the arrangement of collagen fibers within the sclera. A second harmonic generation (SHG) microscope has been used to image the collagen fibers of unstained scleral punches from the posterior part of chicken eyes. We aimed to analyze the fibrous scleral tissue and quantify the changes in collagen organization in relation to the extent of induced deprivation myopia.
View Article and Find Full Text PDFPhase imaging techniques are pivotal for achieving high-contrast visualization of unstained biological specimens in vitro, which is typically not applicable in narrow spaces. Recently, multimode fiber (MMF) has shown promise in enabling high-resolution in vivo endoscopy in biological research. Herein, we introduce a novel, to the best of our knowledge, phase imaging microscopy technique employing a single multimode fiber, showcasing remarkable capabilities in high-contrast imaging and quantitative shape reconstruction through frequency-domain modulation.
View Article and Find Full Text PDFGeometric characteristics of stromal collagen fibres in breast cancer using differential interference contrast microscopy.
J Microsc
February 2025
Academic Unit for Translational Medical Sciences, School of Medicine, University of Nottingham, Nottingham, UK.
Breast cancer (BC) is characterised by a high level of heterogeneity, which is influenced by the interaction of neoplastic cells with the tumour microenvironment. The diagnostic and prognostic role of the tumour stroma in BC remains to be defined. Differential interference contrast (DIC) microscopy is a label-free imaging technique well suited to visualise weak optical phase objects such as cells and tissue.
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