AI Article Synopsis

  • Thiobacillus novellus can grow using oxalate, formate, formamide, and methanol as its only carbon and energy sources.
  • Extensive growth on methanol requires additional nutrients like yeast extract or vitamins.
  • Different metabolic pathways are used for the various substrates, with oxalate being utilized heterotrophically and formate/formamide autotrophically, highlighted by specific enzyme detections.

Article Abstract

Thiobacillus novellus was able to grow with oxalate, formate, formamide, and methanol as sole sources of carbon and energy. Extensive growth on methanol required yeast extract or vitamins. Glyoxylate carboligase was detected in extracts of oxalate-grown cells. Ribulose bisphosphate carboxylase was found in extracts of cells grown on formate, formamide, and thiosulfate. These data indicate that oxalate is utilized heterotrophically in the glycerate pathway, and formate and formamide are utilized autotrophically in the ribulose bisphosphate pathway. Nicotinamide adenine dinucleotide-linked formate dehydrogenase was present in extracts of oxalate-, formate-, formamide-, and methanol-grown cells but was absent in thiosulfate- and acetate-grown cells.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC235443PMC
http://dx.doi.org/10.1128/jb.131.2.389-398.1977DOI Listing

Publication Analysis

Top Keywords

formate formamide
16
oxalate formate
8
formamide methanol
8
thiobacillus novellus
8
ribulose bisphosphate
8
formamide
4
methanol metabolism
4
metabolism thiobacillus
4
novellus thiobacillus
4
novellus grow
4

Similar Publications

Enhancing CO reduction with formamide-Ni@TiO catalyst.

J Environ Sci (China)

July 2025

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Formamide condensation with Ni can generate the NC structure, widely recognized as an efficient catalyst for electrocatalytic CO reduction reaction (CORR). To improve the utilization efficiency of Ni atoms, we introduced metal oxides as substrates to modulate the growth of a formamide-Ni (FA-Ni) condensate. FA-Ni@TiO demonstrated 2.

View Article and Find Full Text PDF
Article Synopsis
  • Carbon-one-unit (C1) feedstocks like formate and formamide are important for producing organic molecules, and they're cleaner alternatives to carbon monoxide from fossil fuels.
  • New methods are emerging to transform carbon dioxide (CO), a greenhouse gas, into these valuable feedstocks, addressing the need for carbon recycling and sustainability.
  • The formylmethanofuran dehydrogenase (FMD) enzyme, found in certain archaea, effectively captures and processes CO without high energy costs, playing a crucial role in the carbon cycle and potentially aiding in renewable energy solutions.
View Article and Find Full Text PDF

High-strength wastewater containing elevated levels of chloride salt and N, N-dimethylformamide (DMF) solvent was collected from manufacturing of sunscreen cream (for UVA/UVB protection) at a cosmetic factory. In evaporation process, precipitates, formed due to the high chloride content (around 160 g/L), clog the pipeline, seriously reducing the treatment efficiency. This study aimed to develop a two-stage process integrating chemical precipitation and electrochemical oxidation to specifically remove the concentrated chloride salt and organic compounds (COD >100 g/L).

View Article and Find Full Text PDF

This study demonstrated the effects of the sludge type and inoculation method on the N,N-dimethylformamide degradation pathway and associated microbial communities. The sludge type is critical for DMF metabolism, with acclimatized aerobic sludge having a significant advantage in terms of DMF metabolism performance, whereas acclimatized anaerobic sludge has a reduced DMF metabolism capacity. Metagenomic revealed increased abundances of Methanosarcina, Pelomona and Xanthobacter in the adapted anaerobic sludge, suggesting that anaerobic sludge can utilize the methyl products produced by DMF metabolism for growth.

View Article and Find Full Text PDF

Ruthenium(II) polypyridyl complexes (RPCs) that emit from triplet metal-to-ligand charge transfer (MLCT) states find a wide variety of uses ranging from luminophores to potential anti-cancer or anti-bacterial therapeutics. Herein we describe a greener, microwave-assisted synthetic pathway for the preparation of homoleptic [Ru(N^N)] and bis-heteroleptic [Ru(N^N)(N'^N')] type complexes. This employs the bio-renewable solvent Cyrene™, dihydrolevoglucosenone, as a green alternative to ,'-dimethylformamide (DMF) in the synthesis of Ru(N^N)Cl intermediate complexes, obtaining comparable yields for N^N = 2,2'-bipyridine, 1,10-phenanthroline and methylated derivatives.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!