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A new way of the Coombs test using flow cytometry-based assay to assess erythrocytes-bound IgG antibodies in the human and rabbit model.
Int J Immunopathol Pharmacol
January 2025
College of Medical Laboratory, Dalian Medical University, Dalian, Liaoning, China.
The Coombs test is important in hematology for detecting erythrocyte-bound IgG antibodies or in serm through agglutination methods, but its sensitivity and specificity are limited. Flow cytometry provides a more precise and sensitive alternative for quantitatively assessing RBC-bound IgG antibodies. This assessment is crucial for evaluating the risk of hemolytic reactions and ensuring safe transfusions.
View Article and Find Full Text PDFOptimization of a Conventional Assay into SYBR Green Real-Time PCR for the Detection of the Nc5 Segment from Neospora caninum.
Acta Parasitol
January 2025
Department of Veterinary Medicine, Federal University of Paraná, Rua Dos Funcionários, 1540, Curitiba, Paraná, 80035-050, Brazil.
Purpose: The aim of the present study was to establish a SYBR Green-based real-time PCR assay for detection of the Nc5 segment from the Neospora caninum genome.
Methods: The oligonucleotides sequences targeting the Nc5 gene previously reported and designed in-house were validated. Two Primer sets were evaluated and tested in four different combinations.
Proteomic study of the inhibitory effects of tannic acid on MRSA biofilm.
Front Pharmacol
December 2024
School of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, China.
Introduction: The mechanism of tannic acid (TA) intervention on methicillin-resistant (MRSA, USA 300) biofilm formation was explored using proteomics.
Methods: The minimum inhibitory concentration (MIC) of TA against the MRSA standard strain USA 300 was determined by two-fold serial dilution of the microbroth. The effects of TA were studied using crystal violet staining.
Optical density (OD) is an important indicator of microbial density, and a commonly used variable in growth curves to express the growth of microbial culture. However, OD values show a linear relationship with bacterial concentration only at low concentrations. When the cell density is high, the relationship loses linearity, and serial dilution is needed to obtain readings of better accuracy.
View Article and Find Full Text PDFThe Standardization and Quantification of Nuclear Factor Kappa B p65 by Real-Time Quantitative Polymerase Chain Reaction.
Cureus
November 2024
Microbiology, Retired-Private Practice, Chennai, IND.
The accurate quantification of nuclear factor Kappa B p65 (NF-κB p65) is critical for understanding inflammatory mechanisms, especially in HIV-1 infected individuals, where NF-κB p65 contributes to chronic immune activation. Conventional methods such as enzyme-linked immunosorbent assay (ELISA) and western blotting are limited in terms of sensitivity and reproducibility. This study aimed to devise a standardized real-time quantitative polymerase chain reaction (RT-qPCR) assay for NF-κB p65 using specifically designed primers and a probe.
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