Differences between porin isolated from Rhodobacter capsulatus B10 and 37b4.

Porin was isolated from Rhodobacter (Rb.) capsulatus wild type B10, as well as from Rb. capsulatus 37b4 as a control. The porin from Rb. capsulatus B10 shows significant differences to that of Rb. capsulatus 37b4 in N-terminal sequence, amino acid composition and molecular mass. The apparent molecular mass of the purified porin from Rb. capsulatus B10 is about 28 kDa by SDS-PAGE, whereas the native porin trimer migrates at 75 kDa. For Rb. capsulatus 37b4 the molecular mass of the momomer is about 30 kDa and for the trimer about 76 kDa. These differences may be related to the morphological differences between the two wild type strains. Rb. capsulatus B10 has an extracellular capsule whereas Rb. capsulatus 37b4 is capsuleless. The native proteins from both wild types showed similar single channel conductance measurements in black lipid membranes. The B10 porin has been crystallised using the detergent beta-d-octyl-gluco-pyranoside. The crystals diffracted to 3 A and belong to the orthorhombic space-group P212121. The crystal packing could be elucidated by molecular replacement.