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Engineering CjCas9 for Efficient Base Editing and Prime Editing.
CRISPR J
December 2024
Gene Editing Center, School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Article Synopsis
- The CRISPR-Cas9 system is utilized for gene therapy but faces challenges with packaging the Cas9 protein in viral vectors, limiting its clinical use.
- Researchers developed enhanced versions of base editors (enCjBEs and enCjABEs) using a smaller Cas9 protein (CjCas9) which showed improved editing efficiencies for converting specific DNA bases.
- New variants like SsenCjPE significantly increase prime editing efficiency and can be further improved by combining with other proteins, making them promising tools for both research and medical applications.
Ubiquitylation of nucleic acids by DELTEX ubiquitin E3 ligase DTX3L.
EMBO Rep
October 2024
Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
The recent discovery of non-proteinaceous ubiquitylation substrates broadened our understanding of this modification beyond conventional protein targets. However, the existence of additional types of substrates remains elusive. Here, we present evidence that nucleic acids can also be directly ubiquitylated via ester bond formation.
View Article and Find Full Text PDFStructural basis of tRNA recognition by the widespread OB fold.
Nat Commun
July 2024
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD, USA.
Article Synopsis
- - The oligonucleotide/oligosaccharide-binding (OB)-fold is crucial for recognizing various biomolecules and plays significant roles in processes like genome maintenance and protein synthesis.
- - A study reveals the 2.6 Å co-crystal structure of a specific bacterial protein (Trbp111) bound to tRNA, showing it recognizes tRNAs mainly by their 3' ends, particularly the conserved 3' CA dinucleotide.
- - The research highlights a new understanding of how ancient protein folds recognize tRNA, which may enhance our knowledge of tRNA-related functions like aminoacylation and localization.
Optical Label-Free Aptasensor Based on Weak Value Amplification for Real-Time and Ultrasensitive Detection of IgE.
ACS Sens
July 2024
Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.
Allergy is a prevalent disease, and the potential allergic population is expanding with industrialization and changes in people's living standards. Serum immunoglobulin E (IgE) level is one of the critical indicators for determining allergy. Here, we proposed a simple, real-time monitoring, low chip cost, label-free aptamer biosensing strategy based on weak value amplification (WVA) for the quantitative detection of IgE in serum samples, enabling early and accurate diagnosis of allergic or hypersensitive patients.
View Article and Find Full Text PDFGlobal kinetic mechanism describing single nucleotide incorporation for RNA polymerase I reveals fast UMP incorporation.
Biophys Chem
September 2024
Department of Chemistry, University of Alabama at Birmingham, Birmingham, AL 35294, USA. Electronic address:
RNA polymerase I (Pol I) is responsible for synthesizing ribosomal RNA, which is the rate limiting step in ribosome biogenesis. We have reported wide variability in the magnitude of the rate constants defining the rate limiting step in sequential nucleotide additions catalyzed by Pol I. in this study we sought to determine if base identity impacts the rate limiting step of nucleotide addition catalyzed by Pol I.
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