AI Article Synopsis
- Researchers isolated two new neuropeptides from the brain extracts of Colorado potato beetles, showing similarities to neuropeptide F (NPF).
- The purification process involved reverse phase chromatography and was supported by multiple analytical techniques including Edman degradation and mass spectrometry.
- The identified peptides, named Led-NPF-1 and Led-NPF-2, have specific amino acid sequences and are classified within the invertebrate neuropeptide Y (NPY)-like peptides group.
Article Abstract
Two novel neuropeptides with neuropeptide F (NPF)-like immunoreactivity have been isolated from brain extracts of the Colorado potato beetle. Purification was achieved primarily by use of reverse phase chromatography including initial C-18 Sep-Pak cartridges and 4 subsequent analytical HPLC columns. Combined data from automated Edman degradation, immunochemical analysis, u.v. absorbance and mass spectrometry led to the elucidation of their full primary structures. The deduced sequences are: Ala-Arg-Gly-Pro-Gln-Leu-Arg-Leu-Arg-Phe-NH2 (ARGPQLRLRFamide) and Ala-Pro-Ser-Leu-Arg-Leu-Arg-Phe-NH2 (APSLRLRFamide). On the basis of their primary structure both peptides can be appended to the invertebrate group of neuropeptide Y (NPY)-like peptides, generally referred to as NPFs. We suggest these peptides to be designated Led-NPF-1 and Led-NPF-2.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0965-1748(95)00104-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Silencing Gene with dsRNA of Different Lengths Impairs Larval Development in .
Insects
December 2024
Laboratorio de Biotecnología I, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario S/N Nuevo Campus Universitario, Chihuahua 31125, Chihuahua, Mexico.
In the search for effective strategies to control the Colorado Potato Beetle, RNA interference technology has emerged as a promising method due to its capacity to suppress genes selectively. Factors such as the target gene and double-stranded RNA (dsRNA) length are critical for optimizing gene silencing efficiency. In this study, we designed and synthesized in vitro dsRNAs of varying lengths targeting the gene, which encodes the AChE1 isoform of acetylcholinesterase in the beetle.
View Article and Find Full Text PDFDevelopment of a Duplex Immunocapture Reverse-Transcription Quantitative Polymerase Chain Reaction for Large-Scale Detection of Potato Mop-Top Virus in Dormant Potato Tubers.
Plant Dis
January 2025
Colorado State University, Department of Bioagricultural Sciences and Pest Management, 307 Plant Science Bldg, Fort Collins, Colorado, United States, 80523;
Potato is an important sector to the U.S. economy, and it created over $100 billion in economic activity in 2021.
View Article and Find Full Text PDFMolecular target for sprayable double-stranded RNA-based biopesticide against Amphitetranychus viennensis (Acari, Tetranychidae).
Int J Biol Macromol
December 2024
College of Plant Protection, Shanxi Agricultural University, Shanxi Key Laboratory of Integrated Pest Management in Agriculture, Taiyuan 030031, Shanxi, China. Electronic address:
Amphitetranychus viennensis, a destructive pest mite of fruit plants in Europe and Asia, poses a serious challenge due to its adaptability and resistance to multiple acaricides. RNA interference (RNAi)-based technologies offer a promising alternative to address this emerging issue. In this study, we screened for candidate genes that can be targeted for spray-induced gene silencing (SIGS).
View Article and Find Full Text PDFEffects of elicitors on plant host selection by adult Leptinotarsa decemlineata (Coleoptera: Chrysomelidae).
J Insect Sci
November 2024
Department of Horticulture, Oregon State University, Corvallis, OR, USA.
The Colorado Potato Beetle, Leptinotarsa decemlineata Say, is the principal defoliator of potato crops globally. It is well known for its propensity to rapidly develop resistance. Thus, new control options which are resilient to the pest's resistance capabilities are a critical need.
View Article and Find Full Text PDFMandelonitrile produced by commensal bacteria protects the Colorado potato beetle against predation.
Nat Commun
November 2024
Agriculture Ministry Key Laboratory of Integrated Pest Management on Crops in East China/State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Article Synopsis
- The Colorado potato beetle, a major pest, uses bright colors to signal its toxicity to predators, which comes from toxic compounds that are still being studied.
- Research reveals that the beetle’s toxic hydrogen cyanide (HCN) is produced from mandelonitrile and other compounds by a gut bacterium called Proteus vulgaris Ld01, which also synthesizes these compounds through genes.
- Eliminating this bacterium from the beetle reduces HCN emission and makes them more appealing to predators, as shown in experiments where chickens preferred eating bacteria-deprived larvae over others, demonstrating the importance of gut bacteria in the beetle's defense strategy.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!