AI Article Synopsis
- The study explored how fast the octanucleotide d(TTGGCCAA) reacts with a platinum complex in water using advanced mass spectrometry techniques.
- The researchers measured specific rate constants and found that platination occurs significantly quicker in pure water compared to sodium perchlorate solution.
- They observed a preference for the platinum complex to bond with the 5'-guanine position over the 3'-guanine, providing insights into the selectivity of this chemical reaction.
Article Abstract
The kinetics of the reaction between the octanucleotide d(TTGGCCAA) in the single-stranded form in pure water and the platinum complex [Pt(NH3)3(H2O)]2+ was investigated by electrospray ionization and matrix-assisted laser desorption/ionization (MALDI) mass spectrometries coupled with enzymatic degradation of the adducts. These methods led to the determination of specific rate constants of platination. The global rate constant characteristic of the formation of adducts on each 5'- or 3'-guanine were measured by electrospray ionization analysis. The ratios between the 5'- and 3'-adducts were determined from enzymatic degradation of the final reaction mixture and MALDI analysis. The platination in water is approximately eight times faster than in 0.1 M NaClO4. The selectivity of platination is a factor of 2 in favor of the 5'-guanine, and similar to that observed for the reaction between d(CTGGCTCA) and [Pt(NH3)3(H2O)]2+ in 0.1 M NaClO4.
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| http://dx.doi.org/10.1002/(SICI)1096-9888(199607)31:7<802::AID-JMS358>3.0.CO;2-Y | DOI Listing |
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