The temperature (15 or 30 C) at which Haemonchus contortus larvae were allowed to develop to the infective (3rd) stage and the temperature at which they were subsequently stored (4, 15, or 30 C) had no effect on the occurrence of developmental arrest (larval impasse) of the larvae in infected lambs. If a large inoculating dose (10,000 larvae) was given to the lambs, a smaller percentage of nematodes was retained (14.2%) then if a small dose (1,000 larvae, 25.8% retention) was given, but the percentage of larvae in developmenttal arrest was greater (7.7% of the retained nematodes vs 0.02%). The temperatures of larval development and storage had no effect on the sex ratio of the adults that developed (46.3% male nematodes).
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Physiol Plant
January 2025
KWS SEMILLAS IBÉRICA S.L.U, Finca Las Monjas, Miranda, Murcia, Spain.
Stomatal abundance sets plants' potential for gas exchange, impacting photosynthesis and transpiration and, thus, plant survival and growth. Stomata originate from cell lineages initiated by asymmetric divisions of protodermal cells, producing meristemoids that develop into guard cell pairs. The transcription factors SPEECHLESS, MUTE, and FAMA are essential for stomatal lineage development, sequentially driving cell division and differentiation events.
View Article and Find Full Text PDFG3 (Bethesda)
January 2025
Department of Biology, Duke University, Durham, NC 27708, USA.
Insulin/IGF signaling (IIS) regulates developmental and metabolic plasticity. Conditional regulation of insulin-like peptide expression and secretion promotes different phenotypes in different environments. However, IIS can also be regulated by other, less-understood mechanisms.
View Article and Find Full Text PDFClin Transl Med
January 2025
State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, China.
Background: Numerous pathogenic variants causing human oocyte maturation arrest have been reported on the primate-specific TUBB8 gene. The main etiology is the dramatic reduction of tubulin α/β dimer, but still large numbers of variants remain unexplained.
Methods: Using microinjection mRNA and genome engineering to reintroduce the conserved pathogenic missense variants into oocytes or in generating TUBB8 variant knock-in mouse models, we investigated that the human deleterious variants alter microtubule nucleation and spindle assembly during meiosis.
Chem Biol Interact
January 2025
Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, No.218 Jixi Road, Hefei 230022, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, Anhui Medical University, No.81 Meishan Road, Hefei 230032, China; Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Bengbu Medical University, No.287 Changhuai Road, Bengbu, 233000, China. Electronic address:
3-Nitropropionic acid (3-NP) is a naturally occurring mycotoxin produced by various species of fungi and plants. However, the potential impact of 3-NP exposure on reproductive health remains unclear. To address this gap, we conducted an in vitro study to investigate the toxic effects of 3-NP on the developmental processes of mouse embryos.
View Article and Find Full Text PDFbioRxiv
January 2025
MCB Graduate Program, Cell Biology, and Biochemistry, Brown University, 70 Ship St., Box G-E4, Providence, RI 02903, USA.
Female reproductive senescence results from the regulated depletion of a finite pool of oocytes called the ovarian reserve. This pool of oocytes is initially established during fetal development, but the oocytes that comprise it must remain quiescent for decades until they are activated during maturation in adulthood. In order for developmentally competent oocytes to populate the ovarian reserve they must successfully initiate both meiosis and oogenesis.
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