We have developed a cell-free system of E. coli that enables us to study the in vitro translation of natural mRNA mediated by wild-type or mutant EF-Tu. Various mutant EF-Tu species have been analyzed, one of which, EF-Tu [G222D], appeared to be virtually unable to mediate the translation of natural mRNA. Since this mutant factor is able to participate in translation in vivo by suppressing nonsense and frameshift mutations in cooperation with EF-Tu [A375T], a revision of the generally accepted scheme of the elongation cycle has been proposed (Bosch, L., Vijgenboom, E., & Zeef, L.A.H., 1996, Biochemistry 36).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1006/bbrc.1996.1279 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Macrolactin A Is an Inhibitor of Protein Biosynthesis in Bacteria.
Biochimie
January 2025
Laboratory of Antimicrobial Resistance, Institute of Ecological and Agricultural Biology (X-BIO), Tyumen State University, Tyumen, Russia.
Macrolactin A (McA) is a secondary metabolite produced by Bacillus species. It has been known for its antimicrobial properties since the late 1980s, although the exact mechanism of its antibacterial activity remains unknown. In this study, we have found that McA is an inhibitor of protein synthesis in bacteria.
View Article and Find Full Text PDFApplication of a liposomal subunit vaccine in chickens for reduction of gut colonisation.
J Vet Res
December 2024
Department of Bacterial Genetics, Institute of Microbiology, Warszawa, Poland.
Introduction: are the most common cause of food poisoning, which manifests itself in diarrhoea of varying severity. Additionally, because of the increasing number of people with immune deficiencies, more frequent serious complications of infections are being observed. The main source of infection is the consumption of contaminated poultry meat, which is a consequence of the insufficiency of current hygiene and biosecurity to control or eliminate it from the poultry food chain.
View Article and Find Full Text PDFAssessment of potentially toxic and rare earth elements in surface soils of Dong Nai, Vietnam.
Environ Geochem Health
December 2024
Da Lat Nuclear Research Institute, 01 Nguyen Tu Luc, Da Lat, Lam Dong, 670000, Vietnam.
This study investigates the quantities of Rare Earth Elements (REEs) and Potentially Toxic Elements (PTEs) in Dong Nai Province's surface soils. Atomic Absorption Spectrometry (AAS) and Instrumental Neutron Activation Analysis (INAA) were used to determine element concentrations. To validate the concentration results, established reference materials (NIST 2711 and IAEA Soil-7) were used.
View Article and Find Full Text PDFIdentification of an antibiotic from an HTS targeting EF-Tu:tRNA interaction: a prospective topical treatment for MRSA skin infections.
Appl Environ Microbiol
December 2024
Department of Microbiology, Biochemistry, & Molecular Genetics, Rutgers New Jersey Medical School, Newark, New Jersey, USA.
Because of the urgent need for new antibiotics to treat drug-resistant bacterial pathogens, we employed an assay that rapidly screens large quantities of compounds for their ability to interfere with bacterial protein synthesis, in particular, the delivery of amino acids to the ribosome via tRNA and elongation factor Tu (EF-Tu). We have identified a drug lead, named MGC-10, which kills Gram-positive bacteria, including methicillin-resistant (MRSA), with a MIC of 6 µM, while being harmless to mammalian cells in that concentration range. The antibacterial activity of MGC-10 was broad against over 50 strains of antibiotic-resistant samples obtained from hospital infections, where MGC-10 inhibited all tested strains of MRSA.
View Article and Find Full Text PDFLanthanide binding peptide surfactants at air-aqueous interfaces for interfacial separation of rare earth elements.
Proc Natl Acad Sci U S A
December 2024
Department of Chemical Engineering, The City College of New York, New York, NY 10031.
Rare earth elements (REEs) are critical materials to modern technologies. They are obtained by selective separation from mining feedstocks consisting of mixtures of their trivalent cation. We are developing an all-aqueous, bioinspired, interfacial separation using peptides as amphiphilic molecular extractants.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!