The development of oxygen-sensitive paramagnetic materials is being actively pursued because of their potential applications for in vivo electron paramagnetic resonance (EPR) oximetry. Among these materials, fusinite is of particular interest because of the high sensitivity of the EPR linewidth to the partial pressure pO2. Although this material has led to a number of very useful results in experimental systems, its potential use in humans is limited by the need to prove that it will not cause deleterious effects. The strategy used in this study to optimize the biocompatibility of the oxygen-sensitive materials was to prepare small silicon implants containing the fusinite. The use of silicon permits the diffusion of oxygen inside the implant while the material does not have contact with the biological environment. Radiosterilization did not affect the pO2 sensitivity of the material. The feasibility of performing pO2 measurement was verified in vivo by periodically inducing ischemia in the gastrocnemius muscle of mice over a period of 6 weeks.
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Histological assessment of rodent CNS tissues to EPR oximetry probe material.
Adv Exp Med Biol
October 1997
Department of Medicine, Dartmouth Medical School, Hanover, New Hampshire, USA.
The effects of the paramagnetic oxygen sensing material, lithium phthalocyanine (LiPc) and fusinite were assessed in the brain of Mongolian gerbils and the spinal columns of rats respectively, to determine if there are histologically discernible changes in the tissue surrounding the probe material. This information is essential for the evaluation of the role of EPR oximetry in the measurements of pO2 in the CNS; the technique has great potential value for such measurements because it reports on the pO2 accurately and sensitively and, after the initial placement, measurements can be made repeatedly without invasive procedures or anesthesia. Histologic assessments demonstrated the inert nature of both the fusinite and LiPc EPR probes in rodent CNS tissue over relatively long (2 month) time periods.
View Article and Find Full Text PDFDevelopment of biocompatible implants of fusinite for in vivo EPR oximetry.
MAGMA
March 1996
Laboratory of Medical Chemistry, University of Louvain, Brussels, Belgium.
The development of oxygen-sensitive paramagnetic materials is being actively pursued because of their potential applications for in vivo electron paramagnetic resonance (EPR) oximetry. Among these materials, fusinite is of particular interest because of the high sensitivity of the EPR linewidth to the partial pressure pO2. Although this material has led to a number of very useful results in experimental systems, its potential use in humans is limited by the need to prove that it will not cause deleterious effects.
View Article and Find Full Text PDFIn vivo and in vitro EPR oximetry with fusinite: a new coal-derived, particulate EPR probe.
Magn Reson Med
February 1994
Department of Medicine, University of Illinois, Urbana.
The peak-to-peak line width (LW) of the first derivative electron spin resonance (EPR) spectrum of the coal maceral fusinite is reversibly broadened by O2. The extent of broadening per unit of partial pressure of oxygen (pO2) is unusually large, exceeding that of nitroxides by almost two orders of magnitude. This paramagnetic property of fusinite, combined with its very stable physicochemical properties and low toxicity, is shown to be of utility in the measurement of pO2 in vitro and in vivo.
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