The activity of acetylcholinesterase (AChE), 5'-nucleotidase (NT), adenylate cyclase (AC) in P2 fraction of sensory-motor cortex was studied in right-handed, left-handed and ambidexters rats on the 2-nd and 45-th day after revealing preferable forepaw at taking feedstuff out of horizontal tube (1 case, 10 presentations). By bilateral (averaging right and left hemispheres) values of AChE, NT and AC ambidexters differ from animals with absolute motor preference and right-handed rats differ from left-handed ones (2-nd day). In 1.5 month differences between ambidexter and animals with preferable extremity are revealed by NT and AC activity. Chemical brain asymmetry is revealed for ambidexters (AChE, 45-th day), right-handed rats (NT, 45-th day) and left-handed animals (AC, 2-nd day). Functional importance of biochemical characteristics studied is discussed.
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