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Quantification of Phosphatidylethanol 16:0/18:1 in blood using Supercritical Fluid Chromatography-Tandem Mass Spectrometry.
J Anal Toxicol
March 2025
Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Artillerigatan 12, Linköping 587 58, Sweden.
Phosphatidylethanol (PEth) consists of phospholipids synthesized in erythrocyte cell membranes in the presence of ethanol and serves as a sensitive and specific indicator of alcohol consumption. Further research on PEth formation, degradation, and stability in postmortem (PM) samples would support its routine application in forensic toxicology. A supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS) method was developed and validated to quantify PEth 16:0/18:1 in blood.
View Article and Find Full Text PDFOptimized Protocol For DNA Extraction from Human Whole Blood.
Cell Physiol Biochem
January 2025
Pomeranian University in Słupsk, Institute of Biology, Arciszewski St. 22 B, PL 76-200 Słupsk, Poland.
Background/aims: DNA isolation is the initial process in genetic research. The product is used in many PCR reactions (PCR-RFLP, Real-Time PCR, multiplex PCR). That is why it is important to optimize DNA isolation protocol to obtain a good quality of DNA.
View Article and Find Full Text PDFEndothelial Homeostasis Under the Influence of Alcohol-Relevance to Atherosclerotic Cardiovascular Disease.
Nutrients
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Department of Surgery, University of Rochester Medical Center, Rochester, NY 14642-8410, USA.
Alcohol, in the form of ethyl alcohol or ethanol, is a widely consumed substance with significant implications for human health. Research studies indicate multifaceted effects of alcohol on the cardiovascular system with both protective and harmful effects on atherosclerotic cardiovascular disease (ASCVD), depending on the amount involved and the pattern of consumption. Among the critical components of the cardiovascular system are endothelial cells which line blood vessels.
View Article and Find Full Text PDFBlack Gram ( L.) Husk as a Source of Bioactive Compounds: An Evaluation of Starch Digestive Enzyme Inhibition Effects.
Foods
February 2025
Department of Product Development, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
This research recovered bioactive compounds from black gram husk (BGH, a by-product of sprout processing) using different ethanol concentrations and maceration times. Based on the results, the highest phenolic and saponin contents were recovered using an 80% ethanolic solution for 3 h, with the extract having both antioxidant and starch digestive enzyme inhibition effects. The major bioactive compounds present in the extract were gallic acid, gentisic acid, ferulic acid, and vitexin.
View Article and Find Full Text PDFImmunomodulatory Potential of 6-Gingerol and 6-Shogaol in -Fermented Extract on Murine Macrophages.
Int J Mol Sci
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Department of Companion and Laboratory Animal and Science, and Leaders in INdustryuniversity Cooperation 3.0 (LINC 3.0) Project by Ministry of Education, Kongju National University, Yesan 32439, Republic of Korea.
In this study, we aimed to investigate whether the physiological activity of ethanol extracts of was improved after fermentation with strains KCTC 3108 (FLP8) and KCL005 (FLP9). Total polyphenol and flavonoid content was substantially increased after fermentation with FLP8 and FLP9 for 48 h and 24 h, respectively, compared with the unfermented control. The 6-gingerol content was significantly increased in FLP9 after 24 h of fermentation, whereas in FLP8, it remained comparable to pre-fermentation levels.
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