The production and metabolism of prostaglandin (PG) D2 in cultured chick retinal pigment epithelial (RPE) cells were studied. The production of PGD2 was evaluated using thin layer chromatography (TLC) after labeling the RPE cells with 1-14Carachidonic acid (AA) and radioimmunoassay (RIA). The metabolism was evaluated by measuring the activity of 15-hydroxyprostaglandin dehydrogenase. The conversion of AA to PGD2 was detected by TLC, and this conversion was inhibited with indomethacin (10(-5)M). In a steady state, 198 +/- 50 pg/6 x 10(5) cells/2 hours of PGD2 was detected by RIA. The amount of PGD2 was greater than that of both PGE2 and PGF2 alpha. The nicotinamide adenine dinucleotide phosphate (NADP)-linked 15-hydroxyprostaglandin dehydrogenase activity was detected in the 10,000 g supernatant of the RPE cells. These results demonstrated that cultured chick RPE cells produce and metabolize PGD2.
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