Acetylcholinesterase (AChE) is an enzyme that appears in several molecular forms. Individual AChE molecular forms can be isolated and identified on the basis of their velocity sedimentation profiles. We have improved an existing method for analyzing AChE sedimentation profiles with a computer aided data acquisition and analysis system. The software facilitates measurements of AChE activity of individual molecular forms and comparison among AChE velocity sedimentation profiles. In practice, our program increased precision and reduced the time of AChE velocity sedimentation profile analysis. The software can also be applied to velocity sedimentation analysis of other proteins. We plan to upgrade the program by making it compatible with Microsoft Windows.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0169-2607(96)01719-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Microplastics Settling in Turbid Water: Impacts of Sediments-Induced Flow Patterns on Particle Deposition Rates.
Environ Sci Technol
January 2025
Department of Environmental Systems Science, ETH Zürich, Zürich 8092, Switzerland.
When microplastics (MPs) enter water bodies, they undergo various transport processes, including sedimentation, which can be influenced by factors such as particle size, density, and interactions with other particles. Surface waters contain suspended natural particles (e.g.
View Article and Find Full Text PDFAn analytical study for predicting incipient motion velocity of sediments under ice cover.
Sci Rep
January 2025
State Key Laboratory of Coastal and Offshore Engineering, Dalian University of Technology, Dalian, 116024, China.
This study investigates the critical impact of incipient sediment motion on sediment transport estimation and riverbed evolution prediction. In this research, we examine the effects of ice cover on the vertical distribution of flow velocity, establishing a mathematical relationship between the vertical average flow velocities in open channel and ice-covered flows. This leads to the derivation of a formula for incipient motion velocity under ice cover.
View Article and Find Full Text PDFUnraveling the mechanisms underlying AOM-induced deterioration of the settling performance of algal floc.
Water Res
January 2025
School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou 221116, Jiangsu, China; Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei 430072, China. Electronic address:
The influence of algal organic matter (AOM) on the settling performance of algal flocs remains poorly understood. To address this, we employed fractionation techniques based on molecular weight to isolate different AOM fractions and analyzed their effects on floc structure and settling performance. This involved comparing the concentrations, compositions, potentials, and functional groups of organic matter before and after coagulation-sedimentation.
View Article and Find Full Text PDFCo-Flocculation of Mixed-Sized Colloidal Particles in Aqueous Dispersions Under a DC Electric Field.
Materials (Basel)
December 2024
Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, Gifu 501-1193, Japan.
When an electric field is applied to or removed from colloidal particle aqueous dispersions, a reversible increase in sedimentation velocity of the colloidal particles, referred to as the Electrically Induced Rapid Separation (ERS) effect, is observed. While electrophoresis and other interfacial electrokinetic phenomena under applied electric fields are well-studied, the phenomena of particle aggregation and re-dispersion caused by the application and removal of the field remain largely unexplored despite their significance. Experiments using mixed aqueous dispersions of poly (methyl methacrylate) (PMMA) particles of different sizes revealed that applying an electric field induced the formation of co-flocs involving both large and small particles, significantly enhancing the sedimentation velocity.
View Article and Find Full Text PDFLipid nanoparticles (LNPs) are the most advanced delivery system currently available for RNA therapeutics. Their development has accelerated since the success of Patisiran, the first siRNA-LNP therapeutic, and the mRNA vaccines that emerged during the COVID-19 pandemic. Designing LNPs with specific targeting, high potency, and minimal side effects is crucial for their successful clinical use.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!