It was shown that a suspension culture of Macleaya cordata accumulated benzo(c)phenanthridine alkaloids in the cell biomass. By the composition of the alkaloids and their content the cell culture markedly differed from the intact plant and callus tissue. The benzo(c)phenanthridine compounds in the cell culture were mainly represented by the reduced forms: dihydrosanquinarin and dihydrochelirubin. In the green plant they were mainly represented by the oxidized forms: sanquinarin and chelerithrin. The cell culture practically contained no chelerithrin characteristic of the intact plant.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
The dynamics of capillary flow in an open-channel system featuring trigger valves.
Sci Rep
December 2024
Department of Chemistry, University of Washington, Box 351700, Seattle, Washington, 98195, USA.
Trigger valves are fundamental features in capillary-driven microfluidic systems that stop fluid at an abrupt geometric expansion and release fluid when there is flow in an orthogonal channel connected to the valve. The concept was originally demonstrated in closed-channel capillary circuits. We show here that trigger valves can be successfully implemented in open channels.
View Article and Find Full Text PDFHypoxia drives the formation of lung micropapillary adenocarcinoma-like structure through hypoxia-inducible factor-1α.
Sci Rep
December 2024
Department of Pathology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Micropapillary adenocarcinoma (MPC) is an aggressive histological subtype of lung adenocarcinoma (LUAD). MPC is composed of small clusters of cancer cells exhibiting inverted polarity. However, the mechanism underlying its formation is poorly understood.
View Article and Find Full Text PDFCurrent perspectives on the dynamic culture of mesenchymal stromal/stem cell spheroids.
Stem Cells Transl Med
December 2024
Division of Molecular and Regenerative Prosthodontics, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
Mesenchymal stromal/stem cells (MSCs) are promising candidates for regenerative medicine owing to their self-renewal properties, multilineage differentiation, immunomodulatory effects, and angiogenic potential. MSC spheroids fabricated by 3D culture have recently shown enhanced therapeutic potential. MSC spheroids create a specialized niche with tight cell-cell and cell-extracellular matrix interactions, optimizing their cellular function by mimicking the in vivo environment.
View Article and Find Full Text PDFComparison of the synthesis of the alpha-amylase enzyme by the native strain in immobilized and immersed cells.
Iran J Microbiol
December 2024
Department of Biology, Faculty of Science, Farhangian University, Tehran, Iran.
Background And Objectives: The study focused on the amylase enzyme, widely used in the industrial starch liquefaction process. We looked into the best way to immobilize the native strain , which is the only alpha-amylase-producing bacterium, by trapping it in calcium alginate gel. This is a promising way to increase enzyme output.
View Article and Find Full Text PDFFunctional MRGPRX2 expression on peripheral blood-derived human mast cells increases at low seeding density and is suppressed by interleukin-9 and fetal bovine serum.
Front Immunol
December 2024
KU Leuven Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Group, Leuven, Belgium.
Primary human mast cells (MC) obtained through culturing of blood-derived MC progenitors are the preferred model for the study of MRGPRX2- IgE-mediated MC activation. In order to assess the impact of culture conditions on functional MRGPRX2 expression, we cultured CD34-enriched PBMC from peripheral whole blood (PB) and buffy coat (BC) samples in MethoCult medium containing stem cell factor (SCF) and interleukin (IL)-3, modified through variations in seeding density and adding or withholding IL-6, IL-9 and fetal bovine serum (FBS). Functional expression of MRGPRX2 was assessed after 4 weeks via flow cytometry.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!