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Potential risk of monochorionic dizygotic twin blastocyst formation associated with early laser zona dissection of group cultured embryos.
Fertil Steril
February 2015
Southern California Institute for Reproductive Sciences, Newport Beach, California; Southern California Center for Reproductive Medicine, Newport Beach, California.
Objective: To document the risk of in vitro monochorionic dizygotic twin formation in the implementation of a program of blastocyst biopsy with preimplantation genetic screening (PGS).
Design: Case report.
Setting: Private infertility laboratory.
Variable imprinting of the MEST gene in human preimplantation embryos.
Eur J Hum Genet
January 2013
Division of Reproduction and Early Development, Leeds Institute of Genetics, Health and Therapeutics, University of Leeds, The LIGHT Laboratories, Leeds, UK.
There is evidence that expression and methylation of the imprinted paternally expressed gene 1/mesoderm-specific transcript homologue (PEG1/MEST) gene may be affected by assisted reproductive technologies (ARTs) and infertility. In this study, we sought to assess the imprinting status of the MEST gene in a large cohort of in vitro-derived human preimplantation embryos, in order to characterise potentially adverse effects of ART and infertility on this locus in early human development. Embryonic genomic DNA from morula or blastocyst stage embryos was screened for a transcribed AflIII polymorphism in MEST and imprinting analysis was then performed in cDNA libraries derived from these embryos.
View Article and Find Full Text PDFRelationship of follicular size to the development of intracytoplasmic sperm injection-derived human embryos.
Taiwan J Obstet Gynecol
September 2010
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taitung, Taiwan.
Objective: To compare the embryonic development of oocytes obtained from follicles of different sizes.
Materials And Methods: Oocytes (n = 819) were retrieved from women at 40 years of age or younger during 86 in vitro fertilization cycles and categorized as small, medium, or large based on the estimated volume of follicular fluid at the time of retrieval.
Results: The rates of good quality embryos from the large, medium, and small groups on days 2 and 3 were 76.
Derivation and characterization of novel nonhuman primate embryonic stem cell lines from in vitro-fertilized baboon preimplantation embryos.
Stem Cells Dev
June 2011
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, School of Medicine, University of Texas Health Science Center, San Antonio, Texas 78229, USA.
The development of nonhuman primate (NHP) embryonic stem cell (ESC) models holds great promise for cell-mediated treatment of debilitating diseases and to address numerous unanswered questions regarding the therapeutic efficacy of ESCs while supplanting ethical considerations involved with human studies. Here we report successful establishment and characterization of 3 novel baboon (Papio cynocephalus) ESC lines from the inner cell mass of intracytoplasmic sperm injection-derived blastocysts. Embryos were cultured in an improved baboon embryo in vitro culture protocol.
View Article and Find Full Text PDFComparison of day 2 tubal embryo transfer with day 3 embryo transfer into the uterus of intracytoplasmic sperm injection derived human embryos.
Taiwan J Obstet Gynecol
September 2006
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, Taiwan.
Objective: To compare the results of embryo transfer into the uterus (ET) performed on day 3 vs. tubal embryo transfer (TET) on day 2 in couples diagnosed with male factor infertility.
Materials And Methods: We collected data from 34 and 110 patients who underwent intracytoplasmic sperm injection (ICSI) followed by either TET on day 2 or ET on day 3 (January 2001-June 2005), respectively.
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