We describe the complete beta-tubulin folding pathway. Folding intermediates produced via ATP-dependent interaction with cytosolic chaperonin undergo a sequence of interactions with four proteins (cofactors A, D, E, and C). The postchaperonin steps in the reaction cascade do not depend on ATP or GTP hydrolysis, although GTP plays a structural role in tubulin folding. Cofactors A and D function by capturing and stabilizing beta-tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. Sequence analysis identifies yeast homologs of cofactors D (cin1) and E (pac2), characterized by mutations that affect microtubule function.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1016/s0092-8674(00)80100-2 | DOI Listing |
Medicine (Baltimore)
January 2025
Nerve Rehabilitation Center, Beijing Rehabilitation Hospital Affiliated to Capital Medical University, Xixia Zhuang, Badachu, Shijingshan District, Beijing, China.
Ischemic stroke is caused by blockage of blood vessels in brain, affecting normal function. The roles of Signal Transformer and Activator of Transcription 1 (STAT1), CASP8, and MYD88 in ischemic stroke and its care are unclear. The ischemic stroke datasets GSE16561 and GSE180470 were found from the Gene Expression Omnibus database.
View Article and Find Full Text PDFJ Neurosurg
January 2025
1Department of Bioengineering, George Mason University, Fairfax, Virginia.
Objective: The complex mix of factors, including hemodynamic forces and wall remodeling mechanisms, that drive intracranial aneurysm growth is unclear. This study focuses on the specific regions within aneurysm walls where growth occurs and their relationship to the prevalent hemodynamic conditions to reveal critical mechanisms leading to enlargement.
Methods: The authors examined hemodynamic models of 67 longitudinally followed aneurysms, identifying 88 growth regions.
Plant Physiol
January 2025
School of Life Science, Ningxia University, Yinchuan 750021, Ningxia, China.
Cold stress severely impacts the quality and yield of grapevine (Vitis L.). In this study, we extend our previous work to elucidate the role and regulatory mechanisms of Vitis amurensis MYB transcription factor 4a (VaMYB4a) in grapevine's response to cold stress.
View Article and Find Full Text PDFPlant Physiol
January 2025
Anhui Key Laboratory for Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University, Hefei, 230036, P.R. China.
Kiwifruit bacterial canker, a highly destructive disease caused by Pseudomonas syringae pv. actinidiae (Psa), seriously affects kiwifruit (Actinidia spp.) production.
View Article and Find Full Text PDFPLoS One
January 2025
School of Economics and Management, Tibet University, Lhasa, Tibet Autonomous Region, China.
This study, grounded in the Total Factor Productivity (TFP) theoretical framework, examines the impact of digital infrastructure on the productivity of Tibet's cultural industry from 2011 to 2021. It aims to uncover how the innovative allocation of production factors can enhance new-quality productivity. The core aspect of new-quality productivity is the improvement of TFP, with digital infrastructure promoting the cultural industry's development by optimizing industrial agglomeration, improving logistics efficiency, and reducing the urban-rural consumption gap.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!