A deletion was introduced into the thymidine kinase (TK) gene of the BHV1 strain Lam and, or, the complete coding region of the glycoprotein E (gE) gene was deleted to reduce virulence and to make serological differentiation possible. The virulence and immunogenicity of these three BHV1 mutants (TK-, gE- and TK-/gE) were studied in specific-pathogen-free calves. Although inactivation of TK strongly reduced the virulence of the Lam strain, deletion of the gE gene alone sufficed to yield complete attenuation of the Lam strain for seven-week-old calves. The three mutants induced protective immunity against disease after challenge with a virulent BHV1 strain. The reduction of virus shedding after challenge was related to the virulence of the various strains. The immunogenicity of the mutants was also evidenced by the reduction of challenge virus shedding after dexamethasone treatment. None of the mutant viruses could be isolated after dexamethasone treatment. The results demonstrate that the gE- and TK-/gE- mutants are good candidates for incorporation in a BHV1 marker vaccine.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0378-1135(95)00137-9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Innovative epitopes in Protein-A an immuno-informatics approach to combat MDR-MRSA infections.
Front Cell Infect Microbiol
January 2025
Department of Haematology and Oncology, Shenzhen Children's Hospital, Shenzhen, China.
Background: Methicillin-resistant (MRSA) poses a significant challenge in clinical environments due to its resistance to standard antibiotics. Protein A (SpA), a crucial virulence factor of MRSA, undermines host immune responses, making it an attractive target for vaccine development. This study aimed to identify potential epitopes within SpA that could elicit robust immune responses, ultimately contributing to the combat against multidrug-resistant (MDR) MRSA.
View Article and Find Full Text PDFEmergence of Fluorescent Glycodots for Biomedical Applications.
ACS Biomater Sci Eng
January 2025
Environment Emission and CRM Section, CSIR-Central Institute of Mining and Fuel Research Dhanbad, Jharkhand, 828108, India.
Carbohydrate-functionalized quantum dots exhibit excellent physical characteristics and enhance the steric interaction with biological cells and tissues. Glycoconjugation of quantum dots promotes aqueous solubility, stability, and reduced immunogenicity. Carbohydrate-protein interactions are involved in various vital processes and provide insight into cellular recognition, cell-to-cell communication, pathogenicity, antigen-antibody recognition, and enzymatic action.
View Article and Find Full Text PDFEgyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering Approach.
Viruses
January 2025
Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
The present study aims to better understand the nature of currently circulating GPV strains and their pathological impact on the immune system during natural outbreaks among different duck breeds in Egypt. For this purpose, 99 ducks (25 flocks) of different breeds, aged 14-75 days, were clinically examined, and 75 tissue pools from the thymus, bursa of Fabricius, and spleen were submitted for virus detection and identification. Clinical and postmortem findings were suggestive of GPV infection.
View Article and Find Full Text PDFComparative Analysis of Immune Response Genes Induced by a Virulent or Attenuated Strain of .
Int J Mol Sci
January 2025
Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Jiutepec 62550, Mexico.
RNA-seq technology has been widely used for the characterization of the transcriptome profile induced by several diseases in both humans and animals. In the present study, RNA-seq was used to identify the differential expression of genes associated with the immune response in cattle infected with two different strains of , both derived from the same Mexican field isolate, which exhibit distinct phenotypic characteristics: the virulent strain, capable of producing acute clinical signs, and the attenuated strain, capable of stimulating a protective immune response when used as an immunogen with an efficacy greater than 80%. The differential gene expression analysis performed revealed a total of 620 differentially expressed genes (DEGs).
View Article and Find Full Text PDFElegant and Innovative Recoding Strategies for Advancing Vaccine Development.
Vaccines (Basel)
January 2025
Department of Veterinary Microbiology and Immunology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5E2, Canada.
Recoding strategies have emerged as a promising approach for developing safer and more effective vaccines by altering the genetic structure of microorganisms, such as viruses, without changing their proteins. This method enhances vaccine safety and efficacy while minimizing the risk of reversion to virulence. Recoding enhances the frequency of CpG dinucleotides, which in turn activates immune responses and ensures a strong attenuation of the pathogens.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!