AI Article Synopsis
- - A new method was developed to simultaneously measure evodiamine and rutaecarpine in herbal medicine using high-performance liquid chromatography (HPLC) with a specific ion-pair reagent.
- - The two compounds were successfully separated and analyzed within 60 minutes without interference from other ingredients in the herbal decoctions.
- - The mobile phase for the HPLC included a mixture of water, methanol, acetonitrile, sodium dodecyl sulfate (SDS), and phosphoric acid, optimized for effective elution with a pH of 5.0.
Article Abstract
A simple and precise method was established for the simultaneous determination of evodiamine and rutaecarpine in oriental pharmaceutical decoctions containing Evodia Fruit using high-performance liquid chromatography with sodium dodecyl sulfate (SDS) as an ion-pair reagent. Evodiamine and rutaecarpine were eluted within 60 min without interference from co-existing components using an ODS column and a mixture of water-methanol-acetonitrile-SDS-phosphoric acid (600:330:70:5:0.01, v/v/v/w/v, pH 5.0) as a mobile phase.
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| http://dx.doi.org/10.1248/yakushi1947.116.1_59 | DOI Listing |
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Article Synopsis
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