AI Article Synopsis
- Researchers studied the rat aldolase A gene with two different promoters to understand how genes are expressed differently in time and space in higher organisms by creating transgenic mice.
- The expression of the transgene was found to be specific to certain tissues and regulated by development stages, indicating that its activity depends on the gene's structure rather than where it integrates into the genome.
- They identified three specific DNase I hypersensitive sites near the gene, which were present both in the transgene and the natural gene, suggesting important regulatory roles in gene expression.
Article Abstract
In order to understand the molecular mechanisms of the temporal and spatial differences of gene expression in higher organisms, rat aldolase A gene carrying two distinct promoters was introduced into fertilized eggs and the resulting transgenic mice were analyzed. The transgene expression is tissue-specific and is developmentally regulated. In addition, the expression is regulated in a copy-dependent manner irrespective of where the transgene is integrated, suggesting that a mechanism excluding the effect of the integration site exists within the transgene itself. To explore the conformational change of this gene in the genome, the DNase I hypersensitive sites of the gene were examined. Three sites (DHS-1,2, and 3) were identified upstream and downstream of the gene and these sites were retained in the transgene as well as in the gene observed endogenously.
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| http://dx.doi.org/10.1093/oxfordjournals.jbchem.a124952 | DOI Listing |
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