AI Article Synopsis
- Researchers dissected adult Ancylostoma caninum worms into different parts like glands and intestines, then separated their proteins using Triton X-114 into soluble and insoluble fractions.
- Immunoblotting was conducted using sera from immunized rabbits and infected dogs to identify immunodominant antigens that could be useful for diagnostic tests.
- The study also uncovered hidden antigens in the esophagus and intestine fractions, which may serve as potential vaccine candidates for future trials.
Article Abstract
Adult worms of Ancylostoma caninum were dissected and manually separated into cephalic glands, cervical glands, intestine, esophagus and cuticula. These fractions as well as third stage larvae were fractionated with Triton X-114 into water soluble (hydrophilic), Triton soluble (hydrophobic) and unsoluble proteins. These fractions were characterized by immunoblotting with serum from rabbits immunized either with a pool of cervical, cephalic glands and intestine, or the esophagus fraction as well as with sera from percutaneously infected dogs and rabbits. Immunodominant antigens were found that reacted with dog or rabbit post infection sera and could be suited as antigens in serodiagnostic tests. Hidden antigens were found in the several fractions. Those from esophagus and intestine could be vaccine candidates that will be tested in immunization trials.
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