The PstB protein of the phosphate-specific transport (Pst) system of Escherichia coli bound and hydrolyzed ATP, producing ADP. Urea-treated denatured PstB did not bind ATP. The N-terminal amino acid sequence of the immune serum-precipitable PstB protein was determined, and it corresponded to that deduced from the DNA sequence.
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http://dx.doi.org/10.1128/jb.178.13.3974-3977.1996 | DOI Listing |
Chemosphere
January 2025
Guangxi Key Laboratory of Environmental Processes and Remediation in Ecologically Fragile Regions, Guangxi Normal University, 15 Yucai Road, Guilin, 541004, PR China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, 15 Yucai Road, Guilin, 541004, PR China. Electronic address:
Garlic and chili are widely used as food flavoring agents in food cooking, therefore might be accumulated in large amounts in food waste (FW). The effects of garlic and chili on the dissolution, hydrolysis, acidification and methanation in an anaerobic co-digestion system were investigated during the combined co-digestion of FW and excess sludge (ES). Additionally, the transformation of phosphorus form and microbial metabolism changes during the process were analyzed.
View Article and Find Full Text PDFMicrob Pathog
December 2024
Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China. Electronic address:
Aeromonas schubertii infections has caused severe economic losses in aquaculture in China. In this study, we first induced enrofloxacin (ENR) resistance in A. schubertii strains and then analyzed the mechanisms of drug resistance using transcriptomics and metabolomics.
View Article and Find Full Text PDFCureus
November 2024
Department of Pediatrics, Japanese Red Cross Wakayama Medical Center, Wakayama, JPN.
Kawasaki disease (KD) is a vasculitis mainly affecting children under five, with symptoms such as persistent fever, rash, red lips, strawberry tongue, conjunctivitis, and swollen hands and feet. Diagnosis is based on a fever lasting over five days plus at least four of these symptoms. Treatment includes intravenous immunoglobulin (IVIG) and aspirin to reduce complications, especially coronary artery issues.
View Article and Find Full Text PDFSynth Syst Biotechnol
October 2024
State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, 100071, China.
YpsR, a pivotal regulatory protein in the quorum-sensing (QS) of (), is essential for molecular signaling, yet its molecular mechanisms remain poorly understood. Herein, this study systematically investigates the interactions between YpsR and acyl-homoserine lactones (AHLs), shedding light on the selective mechanism of YpsR to various AHL molecules. Using molecular docking and surface plasmon resonance (SPR) analysis, we confirmed YpsR's binding affinities, with the strongest observed for 3OC6-HSL, which notably inhibited growth.
View Article and Find Full Text PDFAppl Environ Microbiol
June 2023
Department of Biology, Kenyon College, Gambier, Ohio, USA.
bacteria inhabit human and soil environments that show a wide range of pH values. Strains include commensals as well as antibiotic-resistant pathogens. We investigated the adaptation to pH stress in E.
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