To transposon tag the tomato Dwarf (D) gene, a tomato line that carries a T-DNA containing a maize Activator (Ac) transposable element closely linked to D was pollinated with a stock homozygous for the d mutation. Hybrid seedlings were screened for dwarf progeny, and three independent dwarf lines were obtained. Two of these lines showed inheritance of a recessive phenotype similar to that conferred by the extreme dwarf (dx) allele. Variegation for the dwarf phenotype in one of these lines suggested that D had been tagged by Ac. Genomic DNA adjacent to Ac in these two lines was isolated by use of the inverse polymerase chain reaction, and the two insertions mapped approximately 2 kb apart. Partial complementation of d was observed when the corresponding wild-type sequence was used in transformation experiments. A cDNA clone of D was sequenced, and the predicted amino acid sequence has homology to cytochrome P450 enzymes.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC161151 | PMC |
| http://dx.doi.org/10.1105/tpc.8.6.959 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
First report of natural infection by cowpea mild mottle virus on and in China.
Plant Dis
November 2024
Chinese Academy of Agricultural Sciences Institute of Vegetables and Flowers, State Key Laboratory of Vegetable Biobreeding, Beijing, Beijing, China.
Article Synopsis
- - Vigna unguiculata, or cowpea, is a significant vegetable crop in Sanya, Hainan, China, but it faces threats from viral diseases like CPMMV, BCMV, and CPPV2 which negatively impact its growth and photosynthesis.
- - Weeds in the area, such as Sesbania cannabina and Physalis angulata, can act as virus hosts, complicating the situation for cowpea cultivation.
- - In July 2022, researchers collected these weeds, extracted their RNA, and identified a viral DNA sequence similar to CPMMV, indicating a link between the weeds and the viral disease affecting cowpeas.
Viral Diversity in Mixed Tree Fruit Production Systems Determined through Bee-Mediated Pollen Collection.
Viruses
October 2024
Agriculture and Agri-Food Canada, London Research and Development Centre, Vineland Research Station, 4902 Victoria Ave N, Vineland Station, ON L0R 2E0, Canada.
Article Synopsis
- Commercial orchards often have mixed species that can be affected by various viruses, with pollen being a significant pathway for virus transmission.
- Metagenomics approaches were used to analyze pollen and bee samples during the blooming season of apricot, cherry, peach, and apple trees, resulting in the detection of 21 unique viruses, including notable ones like prune dwarf virus and prunus necrotic ringspot virus.
- The study revealed a higher complexity of viruses in pollen samples compared to vegetative tissues, highlighting the importance of monitoring pollination ecosystems for better understanding of viral diversity.
Interactions between Common Bean Viruses and Their Whitefly Vector.
Viruses
October 2024
Embrapa Arroz e Feijão, Santo Antônio de Goiás 75375-000, GO, Brazil.
Common bean ( L.) is a widely cultivated crop, representing an important protein source in the human diet in developing countries. The production of this crop faces serious challenges, such as virus diseases transmitted by the whitefly .
View Article and Find Full Text PDFTissue-Specific Regulation of Vesicular Trafficking Mediated by Rab-GEF Complex MON1/CCZ1 From Solanum chilense Increases Salt Stress Tolerance in Arabidopsis thaliana.
Plant Cell Environ
February 2025
Laboratorio de Genómica Funcional, Instituto de Ciencias Biológicas, Universidad de Talca, Talca, Chile.
Salt stress constrains the development and growth of plants. To tolerate it, mechanisms of endocytosis and vacuolar compartmentalization of Na are induced. In this work, the genes that encode a putative activator of vesicular trafficking called MON1/CCZ1 from Solanum chilense, SchMON1 and SchCCZ1, were co-expressed in roots of Arabidopsis thaliana to determine whether the increase in prevacuolar vesicular trafficking also increases the Na compartmentalization capacity and tolerance.
View Article and Find Full Text PDFMitigating Salt Stress with Biochar: Effects on Yield and Quality of Dwarf Tomato Irrigated with Brackish Water.
Plants (Basel)
October 2024
Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy.
The increase in the frequency and magnitude of environmental stresses poses a significant risk to the stability of food supplies. In coastal areas of the Mediterranean, brackish water has long been considered a limitation on horticultural production. In this scenario, the use of biochar in agriculture could be considered a valuable tool to cope with the deleterious effects of salt stress.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!