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Copper and nickel doped carbon dots for rapid and sensitive fluorescent turn-off detection of bilirubin.
Sci Rep
January 2025
Department of Chemistry, Amrita School of Physical Sciences Coimbatore, Amrita Vishwa Vidyapeetham, Coimbatore, India.
Carbon dots doped with metals and non-metals have gained much popularity due to the enhancement in their optical and electronic properties. In this study, polyethyleneimine-functionalized transition metal (nickel or copper) doped carbon dots (CD, NiCD and CuCD) were synthesized through hydrothermal method. The carbon dots exhibited a blue fluorescence at 470 nm when excited at 350 nm.
View Article and Find Full Text PDFUsing Agaricus bisporus crude extract in distance based 3D microfluidic paper-based analytical device and spectrophotometric analytical procedures for thiols determination.
Talanta
December 2024
Analytical Chemistry Division, Chemistry Department, Lomonosov Moscow State University, 119234, Moscow, Russia. Electronic address:
Novel and simple spectrophotometric and distance based procedures for thiols (L-cysteine, N-acetylcysteine, and glutathione) determination in biological fluids and pharmaceuticals have been proposed based on their inhibitory action on the oxidation of catechol in the presence of Agaricus bisporus crude extract (ABE). The influence of L-glycine, L-alanine, L-proline, L-methionine, L-cystine, ascorbic acid, uric acid, and bilirubin on the thiol determination has been investigated. Uric acid, bilirubin, L-cystine (oxidized thiol), and L-amino acids do not interfere with the determination.
View Article and Find Full Text PDFAn interference in bilirubin detection: Pulmonary marginal zone lymphoma presenting monoclonal cryoglobulin.
Clin Chim Acta
February 2025
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China. Electronic address:
Marginal zone lymphoma (MZL) of the lung is an indolent B-cell lymphoma. The peripheral blood of most patients with pulmonary MZL contains low or undetectable monoclonal immunoglobulin (M protein) levels. In this case, the clinical laboratory discovered that the pulmonary MZL patient not only associated with high concentration of monoclonal IgG-type protein but also exhibited obvious gel formation characteristics that interfered with clinical biochemistry tests.
View Article and Find Full Text PDFcRGD-Conjugated Bilirubin Nanoparticles Alleviate Dry Eye Disease Via Activating the PINK1-Mediated Mitophagy.
Invest Ophthalmol Vis Sci
November 2024
Department of Ophthalmology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, People's Republic of China.
Purpose: The purpose of this study was to evaluate the cytoprotective effect and the mechanism of cRGD-conjugated bilirubin nanoparticles (cNPs@BR) in dry eye disease (DED).
Methods: The binding capacity and cellular uptake of cNPs@BR in human corneal epithelial cells (HCECs) were assessed by immunofluorescence. The anti-inflammation and anti-oxidative stress effects of cNPs@BR were determined by flow cytometry, immunofluorescence, Western blot, chromatin immunoprecipitation (ChIP), and ELISA assay in LPS-stimulated RAW264.
A Method for Compensating Hemoglobin Interference in Total Serum Bilirubin Measurement Using a Simple Two-Wavelength Reflectance Photometer.
Sensors (Basel)
October 2024
Department of Engineering and Architecture, University of Trieste, 34127 Trieste, Italy.
Neonatal hyperbilirubinemia (NH) is a common condition in newborns, with elevated bilirubin levels potentially causing neurological damage or death. Accurate and timely measurements of total serum bilirubin are essential to prevent these outcomes. Direct spectrophotometry, a reliable method for measuring bilirubin, is particularly useful in constrained settings due to its potential for portable low-cost instrumentation.
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