Transfer of MPEG(1900)-DSPE from micellar phase to pre-formed liposomes imparts long in vivo circulation half-life to an otherwise rapidly cleared lipid composition. MPEG(1900)-DSPE transfers efficiently and quickly in a time and temperature dependent manner. There is negligible content leakage and a strong correlation between assayed mol% MPEG(1900)-DSPE, liposome diameter increase, and pharmacokinetic parameters such as distribution phase half-life. Since a biological attribute (liposome clearance rate) can be modified by the insertion process, it suggests a simple and economical way to impart site-specific targeting to a variety of liposome delivery systems. This method is also a convenient way to measure the 'brush' thickness of such conjugates directly.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0014-5793(96)00452-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Selection of DNA aptamers that prevent the fibrillization of α-synuclein protein in cellular and mouse models.
Mol Ther Nucleic Acids
September 2024
Department of Chemistry, Carleton University, Ottawa, ON K1S 5B6, Canada.
A neuropathological hallmark of Parkinson's disease (PD) is the aggregation and spreading of misfolded α-synuclein (αSyn) protein. In this study, a selection method was developed to identify aptamers that showed affinity for monomeric αSyn and inhibition of αSyn aggregation. Aptamer exhibited strong inhibition of αSyn aggregation by transmission electron microscopy and Thioflavin T fluorescence.
View Article and Find Full Text PDFThe membrane insertion of the pro-apoptotic protein Bax is a Tom22-dependent multi-step process: a study in nanodiscs.
Cell Death Discov
July 2024
CNRS, Université de Bordeaux, UMR 5095, IBGC, Bordeaux, France.
Membrane insertion of the pro-apoptotic protein Bax was investigated by setting up cell-free synthesis of full-length Bax in the presence of pre-formed nanodiscs. While Bax was spontaneously poorly inserted in nanodiscs, co-synthesis with the mitochondrial receptor Tom22 stimulated Bax membrane insertion. The initial interaction of Bax with the lipid bilayer exposed the hydrophobic GALLL motif in Hα1 leading to Bax precipitation through hydrophobic interactions.
View Article and Find Full Text PDFLocalization of Intramuscular mRNA Delivery Using Deep Eutectic-Lipid Nanocomposites.
Adv Healthc Mater
August 2024
John A. Paulson School of Engineering and Applied Sciences, Harvard University, 150 Western Ave, Allston, MA, 02134, USA.
Article Synopsis
- mRNA is being explored as a treatment for various diseases, but traditional lipid nanoparticles (LNPs) used for delivery can cause safety and efficacy issues due to systemic exposure.
- A new delivery system using a deep eutectic solvent (cholinium malonate) enables precise control over mRNA expression levels at the injection site, allowing for a 68% increase in local muscle expression while reducing unwanted liver expression by 72%.
- This innovative approach enhances existing LNP formulations without needing complex lipid redesign, improving the targeted delivery of mRNA for potential medical applications.
Synthesis of α,ω-bis-Mercaptoacyl Poly(alkyl oxide)s and Development of Thioether Cross-Linked Liposome Scaffolds for Sustained Release of Drugs.
Molecules
March 2024
Laboratory of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, University of Patras, 26510 Rio Patras, Greece.
With the aim to develop novel scaffolds for the sustained release of drugs, we initially developed an easy approach for the synthesis of α,ω-homobifunctional mercaptoacyl poly(alkyl oxide)s. This was based on the esterification of the terminal hydroxyl groups of poly(alkyl oxide)s with suitably -4-methoxytrityl (Mmt)-protected mercapto acids, followed by the removal of the acid labile -Mmt group. This method allowed for the efficient synthesis of the title compounds in high yield and purity, which were further used in the development of a thioether cross-linked liposome scaffold, by thia-Michael reaction of the terminal thiol groups with pre-formed nano-sized liposomes bearing maleimide groups on their surface.
View Article and Find Full Text PDFLipid nanoparticles for RNA delivery: Self-assembling vs driven-assembling strategies.
Adv Drug Deliv Rev
May 2024
Department of Pharmacy, University of Naples Federico II, Via D. Montesano, 49 80131 Naples, Italy. Electronic address:
Among non-viral vectors, lipid nanovectors are considered the gold standard for the delivery of RNA therapeutics. The success of lipid nanoparticles for RNA delivery, with three products approved for human use, has stimulated further investigation into RNA therapeutics for different pathologies. This requires decoding the pathological intracellular processes and tailoring the delivery system to the target tissue and cells.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!