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METTL7A improves bovine IVF embryo competence by attenuating oxidative stress.
Biol Reprod
January 2025
Department of Animal Sciences, Genetics Institute, University of Florida, Gainesville, FL, USA.
In vitro fertilization (IVF) is a widely used assisted reproductive technology to achieve a successful pregnancy. However, the acquisition of oxidative stress in embryo in vitro culture impairs its competence. Here, we demonstrated that a nuclear coding gene, methyltransferase-like protein 7A (METTL7A), improves the developmental potential of bovine embryos.
View Article and Find Full Text PDFFetal fibroblast heterogeneity defines dermal architecture during human embryonic skin development.
J Invest Dermatol
January 2025
Centre for Gene Therapy and Regenerative Medicine, King's College London, Guy's Hospital, Great Maze Pond, London, UK; Directors' Unit, EMBL, Meyerhofstr. 1, 69117 Heidelberg, Germany. Electronic address: https://twitter.com/fionamarywatt.
To investigate heterogeneity of fibroblasts in human fetal skin, we analysed published single-cell RNA sequencing data (8 and 16 post conception weeks (PCW)) and performed single-molecule fluorescence in situ hybridisation to map their spatial distribution and predicted dynamic interactions. Clustering revealed 8 fibroblast populations with developmental stage-specific abundance changes. Proliferative cells (MKI67+) were present at all stages.
View Article and Find Full Text PDFEffect of Time-Lapse Incubation System on In Vitro Development of Alpaca Embryos.
Reprod Domest Anim
February 2025
Veterinary Embryology Laboratory, Professional School of Veterinary Medicine, Universidad Nacional de San Antonio Abad del Cusco, Sicuani-Cusco, Peru.
Currently, incubators with a time-lapse system are widely used for in vitro embryo production in several species, however, their effect on alpaca embryo development compared to conventional incubators remains unknown. The aim of this study was to compare early in vitro embryo development in alpacas using a time-lapse incubator system versus a conventional incubator. Ovaries were obtained from a slaughterhouse and 1048 cumulus-oocyte complexes (COCs) were collected and in vitro matured for 26 h in either a time-lapse system (n = 542) or a conventional incubator (n = 542).
View Article and Find Full Text PDFLack of effect of cyclic adenosine monophosphate modulation during prematuration in vitro on development to the blastocyst stage and expression of specific genes associated with lineage commitment.
JDS Commun
January 2025
Department of Animal Sciences and D.H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, FL 32611-0910.
Pharmacological elevation of cyclic AMP (cAMP) of cultured cumulus-oocyte complexes (COC) before or coincident with initiation of maturation has been reported to improve outcomes for various systems for in vitro production of embryos. Here it was hypothesized that artificial elevation of cAMP in the oocyte for a 2-h period of prematuration would improve developmental competence of matured oocytes and result in increased blastocyst yield and altered expression of genes important for embryonic differentiation. Treated COC were cultured for 2 h with dibutyryl cAMP (dbcAMP), a membrane-permeable form of cAMP, and 3-isobutyl-1-methylxanthine (IBMX), which inhibits phosphodiesterases that convert cAMP to ATP.
View Article and Find Full Text PDFInvestigating how reproductive traits in rice respond to abiotic stress.
J Exp Bot
January 2025
Department of Biosciences, University of Milan (UNIMI), Milan, Italy.
Oryza sativa is one of the most important crops and a food source for billions of people. Anthropic global warming, soil erosion, and unstable environmental conditions affect both its vegetative and reproductive growth, and consequently the final yield of its cultivation. The reproductive phase starts with the transition of apical meristem from vegetative to reproductive, which develops into a panicle, proceeds through the differentiation of the floret, and, after fertilization, the filling of the grain.
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