AI Article Synopsis
- The study investigates how G proteins are involved in signaling through 5-HT1P receptors in guinea pig myenteric ganglia.
- Stimulation with serotonin (5-HT) showed increased binding to the G(alpha)o protein, while G(alpha)s, G(alpha)i, and G(alpha)q did not respond.
- The effects of 5-HT were significantly reduced by pertussis toxin treatment, suggesting a strong reliance on G(alpha)o for this process.
- The findings conclude that 5-HT1P receptor signaling in these neurons primarily engages the trimeric G protein Go.
Article Abstract
The role of G proteins in mediating the signal transduction of the guinea pig myenteric ganglionic 5-hydroxytryptamine (5-HT)1P receptors was examined. Stimulation of ganglionic membranes with 5-HT in the presence of [35S]GTPgammaS or [alpha 32P]GTP increased guanine nucleotide binding to G(alpha)o but not to G(alpha)s, G(alpha)i or G(alpha)q in a concentration-dependent fashion. Pertussis toxin pretreatment markedly reduce this 5-HT induced response. Similarly, the 5-HT1P receptor-mediated slowly developing and long-lasting depolarizing response is potentiated by GTPgammaS and is inhibited by GDPbetaS or pertussis toxin. The activation of G(alpha)o by 5-HT also was mimicked by the 5-HT1P agonist, 5-hydroxyindalpine and was blocked by the selective 5-HT1P antagonist, N-acetyl-5-hydroxytryptophyl-5-hydroxytryptophan amide. These data provide compelling evidence to suggest that transmembrane signaling for the 5-HT1P receptors in isolated myenteric ganglia is transduced by the trimeric Go protein.
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